March

2012-3-18 Sun

After discussion in the seminar, 13 students organized the iGEM team and set out to design our project.

2012-3-24 Sat

Our project application were admitted in our school and the registry work started.

The achievements of one week's work:

Muyuan Chen & Xuelong Fan: responsible for the fund application and team registry work.

Xingyu Zhou: designed the bio-sensor based on the asymmetric colony.

Ying Cheng & Zhu Zhu: Searched the information about quorum sensing.

Xiaodan Xu: Read the literature on E.coli's flagella and division.

Peng Cheng & Le Zhou: designed the wiki and logo.

2012-3-25 Sun

Shen Gao: improved the method of cultivation of E.coli.

2012-3-26 Tue

Muyuan Chen: Did some research on light sensor. We voted and named our team SEU_Omega~

2012-3-27 Wed

Filled the application form on the www.igem.org.

2012-3-31 Sat

Xintong Ling: Finished the simulation of diffusion equation in the rectangular case.

April

2012-4-1 Sun

Xintong Ling: finished the diffusion model based on the random containers and made a plan for further models.

2012-4-5 Thur

Muyuan Chen: Made the schedule and experiment program before our summer vacation. Found some information about toggle switch.

Xuelong Fan: Did some research on the light sensor.

Xiaodan Xu: Learnt about how to use NCBI.

2012-4-7 Sat

Had a group meeting and discussed about the future plans.

Xiaodan Xu: Found out the antisense FtsZ gene can be used to control the shape of E.coli's colony.

2012-4-9 Mon

Shen Gao: Choose Pet-32a plasmid for our experiment.

2012-4-12 Thur

Xiaodan Xu: Found a way to verification the primers.

2012-4-13 Fri

Xintong Ling: Finished the simple cell division model.

2012-4-17 Tue

Shen Gao: Learnt about the primer design.

Xingyu Zhou: Designed the quorum sensing part——the AHL system.

Muyuan Chen: Use vectorNTI to analysis plasmid.

2012-4-19 Thur

Xintong Ling:finished the Cellular Automata based on the AHL density.

Xingyu Zhou:Made the expriment plan for the AHL system and found some related kits for his plan.

2012-4-20 Fri

Muyuan Chen: Made the experiment plan for the antisense FtsZ.

Xintong Ling:improved the model of cellular automata.

2012-4-22 Sun

Shen Gao:improved the method of gel extraction and agarose gel electrophoresis.

Xingyu Zhou:Made a summary of AHL sensing system.

2012-4-24 Tue

Xintong Ling & Kai Gui:made the schedule for the further modeling.

Kai Gui:finished the model of biosensor.

2012-4-26 Thur

Xiaodan Xu:Made the experiment plan for antisense FtsZ.

2012-4-29 Sun

Xiaodan Xu:Made a list of the protection base for the restriction sites.

May

2012-5-2 Wed

Xintong Ling: modified the model of biosensor.

2012-5-3 Thur

Xiaodan Xu:Chose the target fragment of FtsZ.

2012-5-7 Mon

Muyuan Chen & Xiaodan Xu: Modified the experiment program--the promoter and the hairpin of the antisense fragment.

2012-5-8 Tue

Ying Cheng: Made the summary of the previous project.

Kai Gui: Finished the model of cellular automata based on the AHL signal diffusion.

2012-5-10 Thur

Our group discussed about the human practice.

Xingyu Zhou:Modified the experiment program of AHL part.

Xintong Ling: Summarise the previous model.

Xiaodan Xu: sorted out the kits list.

2012-5-11 Fri

Xintong Ling:modified the model of cellular automata.

2012-5-12 Sat

Xingyu Zhou:finished the complete AHL experiment program.

2012-5-13 Sun

Xiaodan Xu: Made a list of possibly useful kits.

2012-5-14 Mon

Muyuan Chen: Ordered the paired termini sequence.

2012-5-16 Wed

Xintong Ling & Kai Gui & Jun Zhang: had a meeting talking about the model.

2012-5-22 Tue

Xingyu Zhou: Found out the chemical way of the AHL detection.

2012-5-24 Thur

Xiaodan Xu: transformed the K082034 to make sure the method of Kit usage is correct.

2012-5-27 Sun

Had a group meeting and summarise the work we had done.Made the schedule for the summer vacation and everyone started the preparation for their final examinations.

2012-5-31 Thur Xingyu Zhou: Finished the budget for our project.

June Week 3

2012-6-12 Tue

We got the paired termini fragment in the PUC vector.

2012-6-16 Sat

Muyuan Chen:wrote the experiment program of AHL.(BBa_T9002+F1610)

June Week 4

PCR: FtsZ target fragment

Transform: F1610 R0010

Recovery: I13521

2012-6-19 Tue

Check: F1610 failed; R0010 growed

Digestion: FtsZ1 FtsZ5 paired termini

Transform: F1610

Culture: R0010

Results: AHL failed

2012-6-20 Wed

Check: F1610 failed again

Transform FtsZ1/5+paired termini

Results: AHL failed

2012-6-21 Thur

Check: None

Miniprep plasmids R0010

Transform FtsZ1/5+paired termini

Culture: F1610; C0261; B0015

Others Found our instructor Zuhong Lu and reported our schedule.

2012-6-22 Fri

Check: F1610 FtsZ5+paired termini failed

PCR: Paired termini

Transform: J5526; I13202; T9002

Culture: FtsZ1+paired termini; T9002

Results: AHL failed; We got ftsZ1+termini~

2012-6-23 Sat

Check: J5526 I13202 growed.

Miniprep plasmids T9002

Culture: J5526; I13202

Others Muyuan Chen : Designed the toggle switch. BBa_K415301 BBa_K415300

June Week 5

Miniprep plasmids: I13202; J5526

Transform: I13202; K415023; K091230

Recovery: Pet32a

Culture: I13202+T9002

2012-6-26 Tue

Check: K415023 failed

Digestion: T9002; I13202

Transform: K415023

Recovery: I13202

Culture: I13202; K091230

Results: Toggle switch failed

2012-6-27 Wed

Digestion: T9002+I13202

Transform: R0082; K415301; M30109; K415023; K091230

Recovery: T9002

2012-6-28 Thur

Check: T9002 failed

Ligation: T9002+I13202

Recovery: J5526

Culture: R0082 K415301 M30109 K415023 K091230

Results: AHL maybe failed.

2012-6-29 Fri

Check: R0082 K415023 failed

Miniprep plasmids: K415301; M30109; K091230

Digestion: Pet32a; paired termini

Ligation: Pet32a+paired termini

Transform: R0082; R0083; R0084

Results: AHL maybe would failed.

2012-6-30 Sat

Check: R0082 R0083 R0084 failed

Miniprep plasmids: Pet32a

Digestion: ftsZ +paired termini; Pet32a

Ligation: ftsZ+paired termini+pet32a

Recovery: Pet32a+egfp

Culture: FtsZ1 +Paired termini

Results: Light sensor failed