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Team:Tuebingen/NotebookAppendix
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| - | + | = Appendix = | |
''To complete this report we list all products and software used over the course of our project.'' | ''To complete this report we list all products and software used over the course of our project.'' | ||
| - | + | == Constructs == | |
| - | <table | + | <table class="wikitable"> |
<tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr> | <tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr> | ||
<tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr> | <tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr> | ||
| Line 18: | Line 18: | ||
</table> | </table> | ||
| - | + | == Kits == | |
* Genaxxon Plasmid DNA Purification Mini Prep | * Genaxxon Plasmid DNA Purification Mini Prep | ||
| Line 25: | Line 25: | ||
* Qiagen Plasmid Midi | * Qiagen Plasmid Midi | ||
| - | + | == Chemicals == | |
We needed the following chemicals: | We needed the following chemicals: | ||
| Line 43: | Line 43: | ||
* Agar-Agar | * Agar-Agar | ||
| - | + | == Software == | |
The following list of software was used in the team: | The following list of software was used in the team: | ||
Revision as of 16:42, 22 September 2012
Contents |
Appendix
To complete this report we list all products and software used over the course of our project.
Constructs
| backbone pRS# | part #1 | part #2 | part #3 |
|---|---|---|---|
| 313 | Padh1 | mPR Danio rerio | Tadh1 |
| 313 | Padh1 | mPR Xenopus laevis | Tadh1 |
| 315 | Pfet3 | rox1 | Tadh1 |
| 315 | Pfet3 | mig1 | Tadh1 |
| 316 | Panb1 | lacZ | Tadh1 |
| 316 | Panb1 | luciferase | Tadh1 |
| 316 | Psuc2 | lacZ | Tadh1 |
| 316 | Psuc2 | luciferase | Tadh1 |
Kits
- Genaxxon Plasmid DNA Purification Mini Prep
- Genaxxon Gel Extraction Mini Prep
- Genaxxon PCR DNA Purification Mini Prep
- Qiagen Plasmid Midi
Chemicals
We needed the following chemicals:
- Ampicillin
beta-lactam antibiotic - Agarose
Polysaccharide, major component of Agar - Dimethylsulfoxid (DMSO)
organic solvent - Acetic Acid
- Ethylenediaminetetraacetic acid (EDTA)
- Ethanol
- TRIS
buffer solution for enzymes - Nucleoside triphosphate
- Trypton
- Isopropanol
- Isopropyl-β-D-thiogalactopyranosid (IPTG)
- LB-medium
used for growth of E.coli - Agar-Agar
Software
The following list of software was used in the team:
- [http://ugene.unipro.ru/ Unipro UGENE]
UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
- [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
All our primers were designed using Vector NTI which is also used by our advisors.
- [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
- [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
- [http://drive.google.com Google Drive]
Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
