From 2012.igem.org

Revision as of 15:10, 22 September 2012

Plasmids injected:

1. pCS2+ GFP-aid: contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP (Green Fluorescent Protein)(Nishimura et al., 2009), this Biobrick created by our team is BBa_K812010, and it was integrated into our Eucaryotic plasmid BBa_K812000 .
Number of Plasmids injected: ~ 3.78E+7


2. pCS2+ citrine: contains the constitutive promoter CMV and the fluorescent protein citrin (yellow), the plasmid BBa_K812130 (ready to use in Xenopus) is from our pCS2+ euckaryotic plasmid BBa_K812000 with the YFP (with the Kozak sequence) BBa_K812030.
Number of Plasmids injected: ~ 4.38E+7


3. pCS2+ mCFP: contains the constitutive promoter CMV and the fluorescent protein mCFP (Cyan Fluorescent Protein). the plasmid BBa_K812130 (ready to use in Xenopus) is from our pCS2+ euckaryotic plasmid BBa_K812000 with the YFP (with the Kozak sequence) BBa_K812032.
Number of Plasmids injected: ~ 4.37E+7

pCS2+ GFP-aid




24h after injection


Eggs are near stage 20, neural fold is visible



z-stack of the embryo

Some eggs expressed GFP-aid and some others not, it means that the injection did not work for some eggs or the eggs do not express GFP-aid



48h after injection



Embryos are at stage 34-38 and move by intermittence




The expression of GFP-aid is localized in different tissue for each tadpole, in spite of the promoter is constitutive (CMV). We can think that the plasmid does not diffuse in the eggs because of the vitellus viscosity.



3 days after injection


Embryos are at stage 41-42 and swim



z-stack pictures: pCS2+ CMV_GFP-aid, GFP expression is not in same tissue between tadpoles: for example the tadpole on the left picture bones of the tail produced GFP, on the right picture the GFP expression is localized in the skin. The only part of the tadpole moving is the heart beatting



pCS2+ citrine





pCS2+ mCFP

References: