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Team:Osaka/week8
From 2012.igem.org
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# Transformation of ligation products into DH5α <i>E.coli</i> | # Transformation of ligation products into DH5α <i>E.coli</i> | ||
# Transfer to liquid culture | # Transfer to liquid culture | ||
| + | # Tolerance assay(140,150) | ||
==September 17 (Mon)== | ==September 17 (Mon)== | ||
Revision as of 09:42, 20 September 2012
September 16 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction :161,162
- Ligation
- 163(C):161(upstream)+151(downstream)+1-3A(vector)
- 164(C):161(upstream)+152(downstream)+1-3A(vector)
- 165(C):162(upstream)+151(downstream)+1-3A(vector)
- 166(C):162(upstream)+152(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
- Transfer to liquid culture
- Tolerance assay(140,150)
September 17 (Mon)
- Transfer to liquid culture:163~166,
September 18 (Tue)
- Miniprep of yesterday's culture:163~166
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformation of plasmid DNA into E.coli HST04 161~166
September 19 (Wed)
- Transfer to liquid culture:161~166,
September 20 (Thu)
- Preparation of glycerol stock of cell culture:161~166
