Team:Tuebingen/NotebookAppendix

From 2012.igem.org

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(Constructs)
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pRS mit jeweiligen Inserts
pRS mit jeweiligen Inserts
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<table border="solid">
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<tr><th>pRS</th><th>p1</th><th>p2</th><th>p3</th></tr>
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<tr><th>313</th><th>Padh1</th><th>mPR danio rerio</th><th>Tadh1</th></tr>
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<tr><th>313</th><th>Padh1</th><th>mPR Xenopus laevis</th><th>Tadh1</th></tr>
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<tr><th>315</th><th>Pfet3</th><th>rox1</th><th>Tadh1</th></tr>
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<tr><th>315</th><th>Pfet3</th><th>mig1</th><th>Tadh1</th></tr>
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<tr><th>316</th><th>Panb1</th><th>lacZ</th><th>Tadh1</th></tr>
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<tr><th>316</th><th>Panb1</th><th>luciferase</th><th>Tadh1</th></tr>
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<tr><th>316</th><th>Psuc2</th><th>lacZ</th><th>Tadh1</th></tr>
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<tr><th>316</th><th>Psuc2</th><th>luciferase</th><th>Tadh1</th></tr>
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</table>
=== Protocols ===
=== Protocols ===

Revision as of 15:46, 19 September 2012



Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

pRSp1p2p3
313Padh1mPR danio rerioTadh1
313Padh1mPR Xenopus laevisTadh1
315Pfet3rox1Tadh1
315Pfet3mig1Tadh1
316Panb1lacZTadh1
316Panb1luciferaseTadh1
316Psuc2lacZTadh1
316Psuc2luciferaseTadh1

Protocols

Kits

  • Genaxxon Plasmid DNA Purification Mini Prep
  • Genaxxon Gel Extraction Mini Prep
  • Genaxxon PCR DNA Purification Mini Prep
  • Qiagen Plasmid Midi


Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar


Software

The following list of software was used in the team:

  • [http://ugene.unipro.ru/ Unipro UGENE]
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
  • [http://drive.google.com Google Drive]
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).