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Team:TU Darmstadt/Materials/LB Bromthymol blue
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== LB-Agar-Bromthymol blue == | == LB-Agar-Bromthymol blue == | ||
=== About === | === About === | ||
| - | Modification of [ LB plates] | + | Modification of [https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB plates] for [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue pH screening]. |
=== Ingredients === | === Ingredients === | ||
* 5 g tryptone | * 5 g tryptone | ||
Revision as of 18:29, 16 September 2012
LB-Agar-Bromthymol blue
About
Modification of LB plates for pH screening.
Ingredients
- 5 g tryptone
- 10 g yeast extract
- 5 g NaCl
- 15 g agar-agar
- ad. 1 L dist. water
- autoclave for 20 min. at 121°C
- wait until the temperature decreased under 60°C and add the prefered antibitic
- Add 15 mL of a bromthymol blue solution
- 1 g bromthymol blue
- 100 mL ethanol
- pour the medium into petri plates (1 L LB-Agar is equal to 40 plates)
- store the plates at 4°C
