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Team:SDU-Denmark/labwork/Protocols/3A
From 2012.igem.org
(Difference between revisions)
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<li>Digestion</li> | <li>Digestion</li> | ||
<i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i> | <i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i> | ||
| - | < | + | <ul type="square"> |
<li>Digest step for Plasmid Backbone</li> | <li>Digest step for Plasmid Backbone</li> | ||
| - | + | ||
| - | + | <b>if sample comes from miniprep</b> | |
| - | + | ||
| - | + | ||
| - | + | ||
</ul> | </ul> | ||
</ol> | </ol> | ||
Revision as of 21:08, 15 September 2012
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Content | Content | Content |
| Content | Content | Content | Content |
3A-Assembly -Ligation of 2 parts into a vector
- PCR step; see PCR protocol
- Digestion BSA is only needed for conventional restriction enzymes, NOT fast digest
- Digest step for Plasmid Backbone if sample comes from miniprep
