Team:SDU-Denmark/labwork/Protocols/3A

From 2012.igem.org

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<li>Digestion</li>
<li>Digestion</li>
<i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i>
<i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i>
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<ol>
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<ol type="i">
  <li>Digest step for Plasmid Backbone</li>
  <li>Digest step for Plasmid Backbone</li>
</ol>
</ol>

Revision as of 20:49, 15 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Content Content Content
Content Content Content Content

3A-Assembly -Ligation of 2 parts into a vector

  1. PCR step; see PCR protocol
  2. Digestion
    3. BSA is only needed for conventional restriction enzymes, NOT fast digest
    1. Digest step for Plasmid Backbone

  • Retrieved from "http://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/3A"