Team:SDU-Denmark/labwork/Protocols/3A

From 2012.igem.org

(Difference between revisions)
Line 281: Line 281:
<li>PCR step; see PCR protocol</li>
<li>PCR step; see PCR protocol</li>
<li>Digestion</li>
<li>Digestion</li>
-
<i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i>
+
<blockquote><i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i></blockquote>
<ol type="a">
<ol type="a">
  <li>Digest step for Plasmid Backbone</li>
  <li>Digest step for Plasmid Backbone</li>

Revision as of 20:05, 15 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID

mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Content Content Content
Content Content Content Content



3A assembly

Ligation of 2 parts into a vector

  1. PCR step; see PCR protocol
  2. Digestion
    3. BSA is only needed for conventional restriction enzymes, NOT fast digest
    1. Digest step for Plasmid Backbone

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