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Team:Osaka/week8
From 2012.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
#* 165(C):162(upstream)+151(downstream)+1-3A(vector) | #* 165(C):162(upstream)+151(downstream)+1-3A(vector) | ||
#* 166(C):162(upstream)+152(downstream)+1-3A(vector) | #* 166(C):162(upstream)+152(downstream)+1-3A(vector) | ||
| - | # Transformation of ligation products | + | # Transformation of ligation products into DH5α <i>E.coli</i> |
# Transfer to liquid culture | # Transfer to liquid culture | ||
| Line 23: | Line 23: | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
| - | # Transformation of plasmid DNA into <i>E. coli | + | # Transformation of plasmid DNA into <i>E.coli</i>: HST04 161~166 |
==September 19 (Wed)== | ==September 19 (Wed)== | ||
Revision as of 04:44, 15 September 2012
September 16 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction :161,162
- Ligation
- 163(C):161(upstream)+151(downstream)+1-3A(vector)
- 164(C):161(upstream)+152(downstream)+1-3A(vector)
- 165(C):162(upstream)+151(downstream)+1-3A(vector)
- 166(C):162(upstream)+152(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
- Transfer to liquid culture
September 17 (Mon)
- Transfer to liquid culture:163~166,
September 18 (Tue)
- Miniprep of yesterday's culture:163~166
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformation of plasmid DNA into E.coli: HST04 161~166
September 19 (Wed)
- Transfer to liquid culture:161~166,
September 20 (Thu)
- Preparation of glycerol stock of cell culture:161~166
