Team:Osaka/week7

September 9 (Sun)

1. Miniprep of yesterday's culture(150,151,152,159,160)
2. Restriction digests of minipreppped parts
3. Electrophoresis
4. Tolerance assay of 144,147(MMC)
5. To liquid culture(144,145,160)

September 10 (Mon)

1. Miniprep of yesterday's culture(160,1-9N)
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Preparation of LB agar plates(Amp)
5. Ligation
   • 160(K):157(upstream)+158(downstream)+1-5A(vector)
6. Transformation of ligation products
7. To liquid culture(146,148)
8. Tolerance assay of 144,145(MMC)

September 11 (Tue)

1. Restriction digests(157,158,159,160,1-9N)
2. Gel electrophoresis of digests
3. Ligation
   • 160(K):157(upstream)+158(downstream)+1-5A(vector)
4. Transformation of ligation products
5. Tolerance assay of 146,148(MMC)

September 12 (Wed)

1. Miniprep of yesterday's culture:160
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Tolerance assay
5. Ligation
   • 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
   • 162(C):159(upstream)+160(downstream)+1-3A(vector)

September 13 (Thu)

1. Colony check
   • 161:Transformed cells didnot grow
2. Sequencing(157,158,159,160)
3. Tolerance assay of 146,148
4. Transformation of ligation products:161
5. To liquid culture(144~148,162)

September 14 (Fri)

1. Miniprep of yesterday's culture:162
2. Restriction digests of minipreppped parts
3. Tolerance assay (Preliminary test of hydrogen peroxide)
4. Gel electrophoresis of digests
5. To liquid culture(161)

September 15 (Sat)

1. Miniprep of yesterday's culture:161
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Gel extraction :161,162
5. Ligation
   • 163(C):161(upstream)+151(downstream)+1-3A(vector)
   • 164(C):161(upstream)+152(downstream)+1-3A(vector)
   • 165(C):162(upstream)+151(downstream)+1-3A(vector)
   • 166(C):162(upstream)+152(downstream)+1-3A(vector)
6. Transformation of ligation products
7. Tolerance assay (Hydrogen peroxide)

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