Team:TU Darmstadt/Materials/tctB162

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(Difference between revisions)
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(→tctB162 Pst1)
Line 46: Line 46:
  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc
  rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc
=== tctB162 Pst1 ===
=== tctB162 Pst1 ===
-
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for removing a Pst1 site.
+
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the elimination of a Pst1 site inside the tctB162 BioBrick.
       5'                            3'
       5'                            3'
  fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
  fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
  rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
  rev: GGCCACACGGGGAACTGGAGATTCAGCGCC

Revision as of 00:28, 14 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]

Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.

tctB162 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc

tctB162 Pst1

Primers for the elimination of a Pst1 site inside the tctB162 BioBrick. 

     5'                             3'
fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162"