Team:TU Darmstadt/Materials/tctA503

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Revision as of 00:11, 14 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA503]

Primers for the construction of a BioBrick containing the large subunit A1 of the tripartite tricarboxylate transporter family.

tctA503 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg
rev: gtttcttcgaattcgcggccgcttctagatggatctgattcagaacttgtcgacg

tctA503 Pst1

Primers for removing a Pst1 site. 

     5'                             3'
fwd: TGGTGCTGATGCCGGCAGTGAAGAACAAGCG
rev: CGCTTGTTCTTCACTGCCGGCATCAGCACCA
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