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Team:Wageningen UR/Journal/week14
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== Lab work == | == Lab work == | ||
| - | ''' | + | ''' Hepatitis B ''' |
| + | |||
| + | ---- | ||
| + | |||
| + | ''30.July'' | ||
* Miniprepping of successful transformants from the transformation (Hep B + BBa_J04500) | * Miniprepping of successful transformants from the transformation (Hep B + BBa_J04500) | ||
* Digestion check of those minipreps | * Digestion check of those minipreps | ||
| - | -> | + | |
| + | ''1.August'' | ||
| + | |||
| + | -> the digestion check was repeated 2 times (once with a longer incubation time and once with a higher amount of DNA in the digestion mixture) until the insert size could be seen on the gel | ||
Revision as of 20:18, 13 September 2012
week 14: 30 july - 5 august
Lab work
Hepatitis B
30.July
- Miniprepping of successful transformants from the transformation (Hep B + BBa_J04500)
- Digestion check of those minipreps
1.August
-> the digestion check was repeated 2 times (once with a longer incubation time and once with a higher amount of DNA in the digestion mixture) until the insert size could be seen on the gel
digestion check 1.August
-> the digestion check confirmed the right insert sizes in the samples they where expected (around 800bp)
TuYV
- The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!
PLRV
The PCR products obtained after Reverse Transcription followed by PCR were used as template to add iGEM prefix and suffix.
