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Revision as of 11:50, 13 September 2012

Contents 1 Methods 2 Protocol 2.1 Medium & Buffer recipes 2.2 CCMV Coat Protein VLP formation 2.3 Hepatitis B Coat Protein VLP formation 2.4 TuYV Coat Protein VLP formation 2.5 PLRV Coat Protein VLP formation 2.6 General Protocol

Methods

Protocol

Medium & Buffer recipes

• LB medium
• SOB medium
• SOC medium
• Disassembly buffer
• Dialysis buffer 10x
• Reassembly buffer
• Virus buffer
• Towbin's electrotransfer buffer 1x
• Towbin's electrotransfer buffer 10x

CCMV Coat Protein VLP formation

• Growing culture
• Dialysis of the VLPs
• Purifing the VLPs

Hepatitis B Coat Protein VLP formation

• Growing culture
• Dialysis of the VLPs
• Purifing the VLPs

TuYV Coat Protein VLP formation

PLRV Coat Protein VLP formation

• RNA isolation from potato leaf material

General Protocol

• Preparation of electrocompetent cells of E. coli
• SDS-Polyacrylamide and native gel electrophoresis
• Dynamic Light Scattering user manual
• French press user manual
• FPLC user manual