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Team:Wageningen UR/Journal/week13
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* PCR on cDNA using coat protein primers | * PCR on cDNA using coat protein primers | ||
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| + | The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars. | ||
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| + | [[File:rna2.jpg]] | ||
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| + | [[File:table1.jpg]] | ||
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| + | We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas). | ||
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| + | [[File:rt-pcr.jpg]] | ||
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| + | Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp. | ||
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Revision as of 09:36, 12 September 2012
week 13: 23 july - 29 july
Not much work was done this week because the team spent four days on going to a three day synthetic biology conference in Munich. We learned a lot there and had a great time!
Office work
Lab work
PLRV
- RNA isolation of potato leaf tissue
- Reverse transcriptase reaction to obtain cDNA
- PCR on cDNA using coat protein primers
The figure below shows the purity of isolated RNA. We isolated RNA of different potato cultivars.
We performed Reverse Transciptase reactions followed by PCR. We used the Revert AID H Minus Reverse Transcription Kit (Fermentas).
Agarose gel of PCR products. Lanes 1 till 4 show the PLRV Coat Protein (CP) bands with the expected size of 627 bp. Lanes 6 till 9 show the PLRV Coat Protein plus Read Through part (CP_RT) with the expected size of 2154 bp.
