Team:TU Darmstadt/Materials/DAPI

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Revision as of 23:52, 11 September 2012

Contents

DAPI

About

DAPI or 4',6-diamidino-2-phenylindole is a fluorescent stain that binds to A/T rich regions in DNA. It is used extensively in fluorescence microscopy. DAPI can pass through an intact cell membrane therefore it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.[1]

Safety

  • Wear nitrile gloves
  • Dispose DAPI accordingly

References

[1] http://en.wikipedia.org/wiki/DAPI

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