Team:TU Darmstadt/Materials/NBT

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== NBT ==
== NBT ==
=== About ===
=== About ===
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Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases alkaline phosphatase]<sup>[1]</sup> The colored product reveals where the antibody is bound, or can be used in immunofluorescence.[1]
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Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the [https://2012.igem.org/Team:TU_Darmstadt/Materials#Phosphatases alkaline phosphatase]. The colored product reveals where the antibody is bound, or can be used in immunofluorescence.<sup>[1]</sup>
=== Application ===
=== Application ===
* 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT)
* 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT)

Revision as of 23:47, 11 September 2012

Contents

NBT

About

Nitro blue tetrazolium chloride is a marker used in the reaction catalysed by the alkaline phosphatase. The colored product reveals where the antibody is bound, or can be used in immunofluorescence.[1]

Application

  • 158 mg Nitro blue tetrazolium chloride (75 mg/mL NBT)
  • 1,475 mL DMF
  • 0,632 mL dist. water

References

[1] http://en.wikipedia.org/wiki/Nitro_blue_tetrazolium_chloride