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Team:TU Darmstadt/Materials/TAE
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=== Ingredients === | === Ingredients === | ||
| - | + | ====For 1L 50x:==== | |
* 242 g Tris-HCl/Tris-base | * 242 g Tris-HCl/Tris-base | ||
* 57 mL 100% acetic acid | * 57 mL 100% acetic acid | ||
* 18,6 g EDTA (Ethylenediaminetetraacetic acid) or 100mL of 0.5M sodium EDTA | * 18,6 g EDTA (Ethylenediaminetetraacetic acid) or 100mL of 0.5M sodium EDTA | ||
Add dH<sub>2</sub>O up to 1L | Add dH<sub>2</sub>O up to 1L | ||
| - | + | ====For 1L 1x:==== | |
* 20ml 50x TAE | * 20ml 50x TAE | ||
Add dH<sub>2</sub>O up to 1L (980mL) | Add dH<sub>2</sub>O up to 1L (980mL) | ||
Revision as of 21:24, 11 September 2012
Contents |
TAE
About
TAE is a commonly used buffer for agarose gel electrophoresis that provides optimal resolution of fragments >4kb in length.
Application
TAE is usually prepared in 50X stocks that are diluted to 1X when needed.
Ingredients
For 1L 50x:
- 242 g Tris-HCl/Tris-base
- 57 mL 100% acetic acid
- 18,6 g EDTA (Ethylenediaminetetraacetic acid) or 100mL of 0.5M sodium EDTA
Add dH2O up to 1L
For 1L 1x:
- 20ml 50x TAE
Add dH2O up to 1L (980mL)
