Team:TU Darmstadt/Materials/Agarose gel loading buffer

From 2012.igem.org

(Difference between revisions)
Adrian E (Talk | contribs)
(Created page with "<html> <link rel="stylesheet" href="https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/css&action=raw&ctype=text/css" type="text/css" /> <div id="TUD"> <div id="...")
Newer edit →

Revision as of 20:09, 11 September 2012

Contents

Agarose gel loading buffer

About

Loading buffer also known as loading dye is mixed with DNA samples for use in agarose gel electrophoresis. It contains a dye to assess the progress of the electrolysis and reagents that render the samples denser than the running buffer (so that the samples sink in the well).[1]

Application

Loading dye usually comes in 5X concentration. To use, add and mix 1/5th volume dye/sample (in order to save material mixing on [http://en.wikipedia.org/wiki/Parafilm Parafilm] is an option). It is crucial that the dye and the expected DNA size do not overlap. Take a look in the spec sheet provided with the stain.

Supplier

...

References

[1] http://openwetware.org/wiki/Agarose_gel_loading_buffer

Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer"