Test if gluten has been metabolized.

From 2012.igem.org

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http://www.sciencedirect.com/science/article/pii/0009898190900824
http://www.sciencedirect.com/science/article/pii/0009898190900824
Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease
Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease
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http://www.pnas.org/content/99/10/6603.full
 
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A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease
 
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1)substrate (peptide) synthesis using ABI Model 433A peptide synthesizer
 
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2)labeling with N-hydroxysuccinimidyl esters. Reactions were carried out on a 20–100-μmol resin scale with a 1–10-fold excess of the label in a minimal volume (1–3 ml) of N-methylpyrrolidinone overnight.
 
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3)Incorporation of the two reporters was accomplished by first labeling the N terminus. The second reporter was then introduced by selectively removing the Lys(Mtt)-protecting group and reaction with the label as described above.
 
http://www.sciencemag.org/content/297/5590/2275.full
http://www.sciencemag.org/content/297/5590/2275.full
Structural Basis for Gluten Intolerance in Celiac Sprue
Structural Basis for Gluten Intolerance in Celiac Sprue
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http://www.sunrisescience.com/Files/pTEF-MF.pdf
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pTEF-MF constitutive yeast vector marked for secretion
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pGAL-MF inducible yeast vector marked for secretion
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http://www.jbc.org/content/263/13/6209.full.pdf
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Prepro-a-factor Has a Cleavable Signal Sequence

Revision as of 17:09, 8 June 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

This page is for Steven's research.

http://eprints.uniss.it/5145/1/Forteschi_M_Study_of_peptidases_involved.pdf 33-mer peptide: LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF

http://www.sciencedirect.com/science/article/pii/0009898190900824 Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease

http://www.sciencemag.org/content/297/5590/2275.full Structural Basis for Gluten Intolerance in Celiac Sprue

http://www.sunrisescience.com/Files/pTEF-MF.pdf pTEF-MF constitutive yeast vector marked for secretion pGAL-MF inducible yeast vector marked for secretion

http://www.jbc.org/content/263/13/6209.full.pdf Prepro-a-factor Has a Cleavable Signal Sequence

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