Team:Tuebingen/ProjectImplementation

From 2012.igem.org

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         <div id="toclick">=== Promoter ===<span><br />
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         <div id="toclick">Promoter<span><br />
[[File:PRS313_Padh1_Danio_Tadh1.png|promoter1]]
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[[File:PRS313_Padh1_Xenopus_Tadh1.png|promoter2]]</span></div>
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        <div id="toclick">Inhibitor<span><br />
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=== Inhibitor ===
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[[File:PRS315_Pfet3_rox1_Tadh1_V3.png|inhibitor1]]
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[[File:pRS315_Pfet3_mig1_Tadh1.png|inhibitor2]]</span></div>
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=== Reporter ===
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[[File:PRS316_Panb1_lacZ_Tadh1.png|reporter1]]
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[[File:pRS316_Psuc2_lacZ_Tadh1.png|reporter2]]
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[[File:PRS316_Psuc2_Luciferase_Tadh1.png|reporter3]]
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== Measurement ==
== Measurement ==

Revision as of 16:26, 10 September 2012




Implementation

Promoter

promoter1

promoter2
Inhibitor

inhibitor1

inhibitor2
Reporter

reporter1 reporter2

reporter3

Measurement

The measurement itself should be limited to an optical one. With this idea in mind, we decided for reporter genes like lacZ and luciferase, because these produce signals which can simply be quantified by optical measurement methods.