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Team:TU Darmstadt/Materials/SOC
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| - | == SOC == | + | === SOC === |
SOC medium is used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_transformation bacterial transformation]. | SOC medium is used in [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_transformation bacterial transformation]. | ||
| - | === Ingredients === | + | ==== Ingredients ==== |
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB] | *[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB] | ||
*20 mM glucose | *20 mM glucose | ||
| - | ===Protocol=== | + | ====Protocol==== |
#Make 1 L of [https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB] media | #Make 1 L of [https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB] media | ||
#After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
Revision as of 23:54, 9 September 2012
SOC
SOC medium is used in bacterial transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Make 1 L of SOB media
- After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution
