Killswitch BioBricks
| Numbers
| Team
| Year
| Trigger
| What it Does
| Where?
|
| <partinfo>BBa_I716462</partinfo> | UC-Berkeley | 2007 | Arabinose promoter | BamHI |
|
| <partinfo>BBa_K512002</partinfo> | USC | 2011 | IPTG, targets GFP gene | CRISPR-mediated GFP plasmid degradation |
|
| <partinfo>BBa_K112808</partinfo> | UC-Berkeley | 2008 | No Promoter | T4 Lysis device | Plate 3, 6G
|
| <partinfo>BBa_K541545</partinfo> | FAGEM | 2011 | IPTG | Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible) |
|
| <partinfo>BBa_K593009</partinfo> | METU-Ankara | 2011 | T7 Promoter | ROSE regulated kill switch, (RNA thermometer, only activates above 42°) |
|
| <partinfo>BBa_K628006</partinfo> | St. Andrews | 2011 | pBad | Protegrin-1 Kill Switch, (Antimicrobial peptide) |
|
| | Strasbourg | 2010 | | I don't know what the heck. |
|
| | Tokyo Metropolitan | 2011 | | Killer Bee e.coli? |
|
| | UNIST Korea | 2011 | | Killed by high and low temps, not if dark. |
|
| | Harvard | 2010 | | Barnase cleaves RNA! |
|
| <partinfo>BBa_I716211</partinfo> | UC Berkeley | 2007 | No Promoter | Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB |
|
| | Hong Kong | 2010 | | Possible new killswitch, not implemented |
|
| <partinfo>BBa_K565001</partinfo> | Valencia | 2011 | ? 12 kb | Colicin G
|
| <partinfo>BBa_K565002</partinfo> | Valencia | 2011 | ? 12 kb | Colicin H
|
| <partinfo>BBa_K565004</partinfo> | Valencia | 2011 | No Promoter | Microcin C51 production device (5 restriction sites) |
|
| <partinfo>BBa_K185004</partinfo> | SJTU-BioX-Shanghai | 2009 | No Promoter | RelE toxin+Double terminator |
|
| | UNICAMP Brazil | 2009 | | All sorts of kill switches, but may not all work in E. coli. |
|
| <partinfo>BBa_K284017</partinfo> | UNICAMP-Brazil | 2009 | Lactate (Yeast) | Lysozyme
|
| <partinfo>BBa_K131000</partinfo> | Calgary | 2008 | SOS Promoter | Colicin 2E | 3, 22A
|
| <partinfo>BBa_K112806</partinfo> | UC Berkeley | 2008 | T7 | T4 Endolysin |
|
| <partinfo>BBa_K302035</partinfo> | Newcastle | 2010 | Sucrose limit | MazEF
|
| <partinfo>BBa_K124014</partinfo> | Brown | 2008 | No Promoter | Mutated Holin Gene (Inconsistent sequencing) |
|
| <partinfo>BBa_K419003</partinfo> | TzuChiU_Formosa | 2010 | pLys | Lysis Cassette - no antiholin |
|
| <partinfo>BBa_K317039</partinfo> | Tokyo-NoKoGen | 2010 | AHL | AHL sensitive lysis cassette |
|
| <partinfo>BBa_K317040</partinfo> | Tokyo-NoKoGen | 2010 | IPTG | IPTG sensitive lysis cassette |
|
| <partinfo>BBa_K299806</partinfo> | Warsaw | 2010 | | MinC cell division inhibitor |
|
| <partinfo>BBa_K150007</partinfo> | Heidelberg | 2008 | Native Operon | Colicin E1 |
|
| <partinfo>BBa_K150010</partinfo> | Heidelberg | 2008 | Native Operon | Colicin E9 |
|
| <partinfo>BBa_K145230</partinfo> | KULeuven | 2008 | HSL-LuxR | ccdb |
|
| <partinfo>BBa_K117009</partinfo> | NTU-Singapore | 2008 | IPTG | Colicin E7 |
|
| <partinfo>BBa_K117000</partinfo> | NTU-Singapore | 2008 | | Lysin (activates Colicin E7) |
|
| <partinfo>BBa_K108001</partinfo> | Tsinghua | 2008 | | Enterobacter phage lysis |
|
Distribution BioBricks
| Location
| Part Name
| Function
| Use
| Other
| Plasmid Resistance
|
| Plate 1 | | | | |
|
| 5P | <partinfo>BBa_P1003</partinfo> | Kan Resistance | Resistance | Full Cassette | "Kan | Amp | Tet"
|
| 7B | <partinfo>BBa_P1004</partinfo> | Chlor Resist | Resistance | Full Cassette | "Kan | Amp"
|
| 14C | <partinfo>BBa_C0261</partinfo> | AHL Synthase | AHL Kill Switch | No Promoter | Amp
|
| 24E | <partinfo>BBa_I13600</partinfo> | CFP reporter | fluorescence | 0 | Amp
|
| Plate 2 | | | | |
|
| 17F | <partinfo>BBa_I20260</partinfo> | GFP reporter | Fluorescence | Strong const. | Kan
|
| 14N | <partinfo>BBa_K117009</partinfo> | Colicin E7 | Kill Switch | Lactose prom | Amp
|
| 14D | <partinfo>BBa_K117000</partinfo> | Lysis gene | Kill Switch | Aids colicin | Amp
|
| 9B | <partinfo>BBa_J09250</partinfo> | GFP reporter | Fluoresence | Strong Const. | Kan
|
| Plate 3 | | | | |
|
| 2D | <partinfo>BBa_I746909</partinfo> | Superfold GFP | Fluoresence | T7 | Amp
|
| 5G | <partinfo>BBa_K137076</partinfo> | H2O2 gen. | Kill Switch | AHL induce | Amp
|
| 6G | <partinfo>BBa_K112808</partinfo> | Lysis Device | Kill Switch | No Promoter | Amp
|
| 13M | <partinfo>BBa_K093012</partinfo> | RFP | Fluorescence | Strong const. | Amp
|
| 14E | <partinfo>BBa_K081018</partinfo> | TetR | Resistance | Strong const. | "Amp | Kan"
|
| 15B | <partinfo>BBa_K112000</partinfo> | Holin | Kill Switch | No promoter | ?Spec
|
| 15D | <partinfo>BBa_K112005</partinfo> | "RBS, Holin" | Kill Switch | No Promoter | ?Spec
|
| 15H | <partinfo>BBa_k112017</partinfo> | lysozyme | Kill Switch | No Promoter | ?Spec
|
| 15J | <partinfo>BBa_K112106</partinfo> | BgIII endonuc. | Kill Switch | No Promoter | ?Spec
|
| 20I | <partinfo>BBa_K124003</partinfo> | Lysis Cassette | Kill Switch | ? | Kan
|
| 20K | <partinfo>BBa_K124014</partinfo> | M S Holin | Kill Switch | No Promoter | "Amp | Kan"
|
| 20M | <partinfo>BBa_K124017</partinfo> | M Lysis cassette | Kill Switch | No Promoter | Kan
|
| 21O | <partinfo>BBa_K106674</partinfo> | GFP | Fluorescence | Med. Const. | ?amp
|
| 22A | <partinfo>BBa_K131000</partinfo> | Colicin E2 | Kill Switch | SOS promoter | "Kan | Amp"
|
| 22C | <partinfo>BBa_K131010</partinfo> | Colicin E2 | Kill Switch | "AHL induce, c GFP" | "Kan | Amp"
|
| 24E | <partinfo>BBa_K112022</partinfo> | Lysis Device | Kill Switch | "No Prom, weak anti protein" | "?Amp | Kan"
|
| Plate 4 | | | | |
|
| 3I | <partinfo>BBa_K398406</partinfo> | Solvent Tolerant | Other (Hex. Tol) | Strong const. | Chlor
|
| 6M | <partinfo>BBa_K112806</partinfo> | T4 Endolysin | Kill Switch | No Prom | Amp
|
| 7C | <partinfo>BBa_K314200</partinfo> | Tse Toxin | Kill Switch | No Prom | Chlor
|
| 8G | <partinfo>BBa_K206000</partinfo> | pBAD Promoter | Promoter | Arabinose ind | Amp
|
| 21G | <partinfo>BBa_K314203</partinfo> | Tse Tox. Ind.Anti | Kill switch | HSL ind immunity | Chlor
|
| Plate 5 | | | | |
|
| 1A | <partinfo>BBa_K515107</partinfo> | Photo-Switch | Death test/Fluor | pTet | Chlor
|
| 12O | <partinfo>BBa_I13604</partinfo> | Con. YFP ind CFP | Fluor | Strong const./lacI | Amp
|
| 14I | <partinfo>BBa_K299806</partinfo> | minC | Kill Switch | No Promoter | Amp
|
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