Team:British Columbia/Interesting Biobricks

From 2012.igem.org

British Columbia - 2012.igem.org

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
<partinfo>BBa_I716462</partinfo> UC-Berkeley 2007 Arabinose promoter BamHI
<partinfo>BBa_K512002</partinfo> USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo> UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
<partinfo>BBa_K541545</partinfo> FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo> UC Berkeley 2007 No Promoter Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> Valencia2011 ? 12 kb Colicin G
<partinfo>BBa_K565002</partinfo> Valencia 2011 ? 12 kb Colicin H
<partinfo>BBa_K565004</partinfo> Valencia 2011 No Promoter Microcin C51 production device (5 restriction sites)
<partinfo>BBa_K185004</partinfo> SJTU-BioX-Shanghai 2009 No Promoter RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K284017</partinfo> UNICAMP-Brazil 2009 Lactate (Yeast) Lysozyme
<partinfo>BBa_K131000</partinfo> Calgary2008 SOS Promoter Colicin 2E3, 22A
<partinfo>BBa_K112806</partinfo> UC Berkeley 2008 T7 T4 Endolysin
<partinfo>BBa_K302035</partinfo> Newcastle 2010 Sucrose limit MazEF
<partinfo>BBa_K124014</partinfo> Brown 2008 No Promoter Mutated Holin Gene (Inconsistent sequencing)
<partinfo>BBa_K419003</partinfo> TzuChiU_Formosa 2010 pLysLysis Cassette - no antiholin
<partinfo>BBa_K317039</partinfo> Tokyo-NoKoGen 2010AHL AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo> Tokyo-NoKoGen 2010 IPTG IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo> Warsaw 2010 MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo> Heidelberg2008 Native Operon Colicin E1
<partinfo>BBa_K150010</partinfo> Heidelberg2008 Native Operon Colicin E9
<partinfo>BBa_K145230</partinfo> KULeuven 2008 HSL-LuxR ccdb
<partinfo>BBa_K117009</partinfo> NTU-Singapore 2008 IPTG Colicin E7
<partinfo>BBa_K117000</partinfo> NTU-Singapore 2008 Lysin (activates Colicin E7)
<partinfo>BBa_K108001</partinfo> Tsinghua 2008 Enterobacter phage lysis


Distribution BioBricks
Location Part Name Function Use Other Plasmid Resistance
Plate 1
5P <partinfo>BBa_P1003</partinfo> Kan Resistance Resistance Full Cassette Kan, Amp, Tet
7B <partinfo>BBa_P1004</partinfo> Chlor Resist Resistance Full Cassette Kan, Amp
14C <partinfo>BBa_C0261</partinfo> AHL Synthase AHL Kill Switch No Promoter Amp
24E <partinfo>BBa_I13600</partinfo> CFP reporter fluorescence 0 Amp
Plate 2
17F <partinfo>BBa_I20260</partinfo> GFP reporter Fluorescence Strong const. Kan
14N <partinfo>BBa_K117009</partinfo> Colicin E7 Kill Switch Lactose prom Amp
14D <partinfo>BBa_K117000</partinfo> Lysis gene Kill Switch Aids colicin Amp
9B <partinfo>BBa_J09250</partinfo> GFP reporter Fluoresence Strong Const. Kan
Plate 3
2D <partinfo>BBa_I746909</partinfo> Superfold GFP Fluoresence T7 Amp
5G <partinfo>BBa_K137076</partinfo> H2O2 gen. Kill Switch AHL induce Amp
6G <partinfo>BBa_K112808</partinfo> Lysis Device Kill Switch No Promoter Amp
13M <partinfo>BBa_K093012</partinfo> RFP Fluorescence Strong const. Amp
14E <partinfo>BBa_K081018</partinfo> TetR Resistance Strong const. Amp, Kan
15B <partinfo>BBa_K112000</partinfo> Holin Kill Switch No promoter ?Spec
15D <partinfo>BBa_K112005</partinfo> "RBS, Holin" Kill Switch No Promoter ?Spec
15H <partinfo>BBa_k112017</partinfo> lysozyme Kill Switch No Promoter ?Spec
15J <partinfo>BBa_K112106</partinfo> BgIII endonuc. Kill Switch No Promoter ?Spec
20I <partinfo>BBa_K124003</partinfo> Lysis Cassette Kill Switch ? Kan
20K <partinfo>BBa_K124014</partinfo> M S Holin Kill Switch No Promoter Amp, Kan
20M <partinfo>BBa_K124017</partinfo> M Lysis cassette Kill Switch No Promoter Kan
21O <partinfo>BBa_K106674</partinfo> GFP Fluorescence Med. Const. ?amp
22A <partinfo>BBa_K131000</partinfo> Colicin E2 Kill Switch SOS promoter Kan, Amp
22C <partinfo>BBa_K131010</partinfo> Colicin E2 Kill Switch AHL induce, c GFP "Kan, Amp
24E <partinfo>BBa_K112022</partinfo> Lysis Device Kill Switch No Prom, weak anti protein Amp, Kan
Plate 4
3I <partinfo>BBa_K398406</partinfo> Solvent Tolerant Other (Hex. Tol) Strong const. Chlor
6M <partinfo>BBa_K112806</partinfo> T4 Endolysin Kill Switch No Prom Amp
7C <partinfo>BBa_K314200</partinfo> Tse Toxin Kill Switch No Prom Chlor
8G <partinfo>BBa_K206000</partinfo> pBAD Promoter Promoter Arabinose ind Amp
21G <partinfo>BBa_K314203</partinfo> Tse Tox. Ind.Anti Kill switch HSL ind immunity Chlor
Plate 5
1A <partinfo>BBa_K515107</partinfo> Photo-Switch Death test/Fluor pTet Chlor
12O <partinfo>BBa_I13604</partinfo> Con. YFP ind CFP Fluor Strong const./lacI Amp
14I <partinfo>BBa_K299806</partinfo> minC Kill Switch No Promoter Amp