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Transition from a prokaryotic bacteria to yeast.
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2. Codon bias. | 2. Codon bias. | ||
| - | I'm still unsure of the process and necessity, but we may need to use different codons in Yeast than are used in E. Coli. So, I've tried to find some info on the codons in both. [http://www.jbc.org/content/257/6/3026.full.pdf Yeast Codon bias]. [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/codon-usage.html E. Coli Codon bias]. [http://mbe.library.arizona.edu/data/1985/0201/2ikem.pdf Direct Comparison of E.coli and Yeast]. | + | I'm still unsure of the process and necessity, but we may need to use different codons in Yeast than are used in E. Coli. So, I've tried to find some info on the codons in both. [http://www.jbc.org/content/257/6/3026.full.pdf Yeast Codon bias]. [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/codon-usage.html E. Coli Codon bias]. [[http://mbe.library.arizona.edu/data/1985/0201/2ikem.pdf Direct Comparison of E.coli and Yeast]. |
[http://www.genscript.com/cgi-bin/tools/rare_codon_analysis This is a tool for analyzing the codon bias of a given protein strand in a specific organism]. | [http://www.genscript.com/cgi-bin/tools/rare_codon_analysis This is a tool for analyzing the codon bias of a given protein strand in a specific organism]. | ||
| - | [http://www.vivo.colostate.edu/molkit/translate/index.html This gives optimized nucleic acid sequence for a given amino acid sequence]. | + | [http://www.vivo.colostate.edu/molkit/translate/index.html Another tool for analyzing the codon bias of a given protein strand in a specific organism]. |
| + | |||
| + | [http://www.encorbio.com/protocols/Codon.htm This gives optimized nucleic acid sequence for a given amino acid sequence]. | ||
3. Secreting enzyme into beer. | 3. Secreting enzyme into beer. | ||
https://static.igem.org/mediawiki/2008/2/27/JHU_0708_paper_ForeignGeneExpression.pdf | https://static.igem.org/mediawiki/2008/2/27/JHU_0708_paper_ForeignGeneExpression.pdf | ||
Revision as of 22:54, 7 June 2012
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Currently I'm looking into 3 aspects of the transition to yeast:
1. Subcloning into a yeast expression plasmid.
2. Codon bias.
I'm still unsure of the process and necessity, but we may need to use different codons in Yeast than are used in E. Coli. So, I've tried to find some info on the codons in both. [http://www.jbc.org/content/257/6/3026.full.pdf Yeast Codon bias]. [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/codon-usage.html E. Coli Codon bias]. [[http://mbe.library.arizona.edu/data/1985/0201/2ikem.pdf Direct Comparison of E.coli and Yeast].
[http://www.genscript.com/cgi-bin/tools/rare_codon_analysis This is a tool for analyzing the codon bias of a given protein strand in a specific organism].
[http://www.vivo.colostate.edu/molkit/translate/index.html Another tool for analyzing the codon bias of a given protein strand in a specific organism].
[http://www.encorbio.com/protocols/Codon.htm This gives optimized nucleic acid sequence for a given amino acid sequence].
3. Secreting enzyme into beer.
https://static.igem.org/mediawiki/2008/2/27/JHU_0708_paper_ForeignGeneExpression.pdf
