Team:TU Darmstadt/Labjournal/Transport

From 2012.igem.org

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===Week 1 (21.-25.05.12)===
===Week 1 (21.-25.05.12)===
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* First [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR colony PCR] of ''Comamonas t.'' for isolation of the following genes: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA 505aa] (1), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB 197aa] (2), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA_503aa] (3), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB 162aa] (4), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808001 tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis agarose gel electrohporesis]
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* First [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR colony PCR] of ''Comamonas testosteroni'' for isolation of the following genes: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA 505aa] (1), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB 197aa] (2), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA_503aa] (3), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB 162aa] (4), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808001 tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis agarose gel electrohporesis]
:https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png
:https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png

Revision as of 19:40, 8 September 2012

Transport

Week 1 (21.-25.05.12)

  • First colony PCR of Comamonas testosteroni for isolation of the following genes: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA 505aa] (1), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB 197aa] (2), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA_503aa] (3), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB 162aa] (4), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808001 tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by agarose gel electrohporesis
W1_1_kolo_pcr.png