Team:TU Darmstadt/Labjournal/Transport

From 2012.igem.org

(Difference between revisions)
Line 40: Line 40:
== Transport ==
== Transport ==
-
===week 1 (21.-25.05.12)===
+
===Week 1 (21.-25.05.12)===
-
* First [[Colony-PCR]] of Comamonas for isolation of the following genes: [[tctA 505aa]] (1), [[tctB 197aa]] (2), [[tctA 503aa]] (3), [[tctB 162aa]] (4), [[tphC 322aa]] (5), 50µl reaction mixture each; analysis of PCR products by [[Agarose gel electrohporesis]]
+
* First [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR colony PCR] of ''Comamonas t.'' for isolation of the following genes: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA 505aa] (1), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB 197aa] (2), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA_503aa] (3), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB 162aa] (4), [tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis Agarose gel electrohporesis]
:https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png
:https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png
-
[[File:W1_1_kolo_pcr.png|250px]
 

Revision as of 12:59, 6 September 2012

Transport

Week 1 (21.-25.05.12)

  • First colony PCR of Comamonas t. for isolation of the following genes: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808004 tctA 505aa] (1), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB 197aa] (2), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808002 tctA_503aa] (3), [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB 162aa] (4), [tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by Agarose gel electrohporesis
W1_1_kolo_pcr.png