Team:Evry/Notebook/w13

From 2012.igem.org

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<h2>Monday 3rd</h2>
<h2>Monday 3rd</h2>
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'''[[
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<u><h5>Construction about a new pCS2+ with RFP insert</u></h5>
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Construction about a new pCS2+ with RFP insert :]]'''
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-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
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<h2>Tuesday 3rd</h2>
<h2>Tuesday 3rd</h2>
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'''[[
 
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'''[[Preparation of a new stock of electrocompetent cells T10]]'''
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<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5>
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Construction about a new pCS2+ with RFP insert :]]'''
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<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5>
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''Following''
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<i>Following</i>
-Transformation by electroporation with T10 cells
-Transformation by electroporation with T10 cells

Revision as of 16:26, 5 September 2012

Weeks:

June July August September October November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI -Purification gel to get only RFP insert ( gel image ) -Extraction of the RFP insert -Ligation with pCS2+

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
Following -Transformation by electroporation with T10 cells -Plates prepared

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