Team:Fatih-Medical

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Revision as of 19:26, 7 September 2012

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CANCEL the CANCER: A SynBio Approach to the Early Diagnosis of Cancer

Our project is mainly based on the early diagnosis of cancer. EpCAM (Epithelial cell adhesion molecule) is a pan-epithelial differentiation antigen overexpressed on the basolateral surface of most carcinomas and Circulating Tumor Cells(CTC); cells which are released into blood in early phases of cancer. Our objective is to fix appropriate antibodies for EpCAM antigens to the E.coli cell wall so that we will be able to detect CTCs before the cancer precipitates its way to metastasis. For the next step, we plan to enhance the detection signal in our bacteria by the means of the quorum sensing mechanism. Finally, to prevent the production of possible undesirable and detrimental genetically modified organisms (GMOs), we aim to induce self-destruction device in our E.coli via emission of light.

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-    Our project is based on the early diagnosis of cancer. Circulating Tumor Cells (CTCs) are the cells released into blood from early phase of the cancer. On the surface of these cells, there are specific antigens called epithelial cell adhesion molecule (EpCAM). Furthermore, the free tumor DNA of the degraded CTC contains specific mutations. Our goal is to place appropriate antibodies for EpCAM antigens and DNA binding transcription activator-like effectors (TALE) proteins into the bacterial cell wall so that we can detect CTCs or the free tumor DNA, which is released by the lysis of the tumor cells and proceeds to the blood. In this way, we will hopefully diagnose cancer before it paves the way to metastasis. To amplify the signal, we want to use the quorum sensing mechanism in our bacteria. Finally, to prevent the production of possible unwilling genetically modified organisms (GMOs), we plan to induce self-destruction in our bacteria via emitting light.
+    Our project is mainly based on the early diagnosis of cancer. EpCAM (Epithelial cell adhesion molecule) is a pan-epithelial differentiation antigen overexpressed on the basolateral surface of most carcinomas and Circulating Tumor Cells(CTC); cells which are released into blood in early phases of cancer. Our objective is to fix appropriate antibodies for EpCAM antigens to the E.coli cell wall so that we will be able to detect CTCs before the cancer precipitates its way to metastasis. For the next step, we plan to enhance the detection signal in our bacteria by the means of the quorum sensing mechanism. Finally, to prevent the production of possible undesirable and detrimental genetically modified organisms (GMOs), we aim to induce self-destruction device in our E.coli via emission of light.
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