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Team:Wageningen UR/Journal/week2
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= week 2: 7 may - 11 may = | = week 2: 7 may - 11 may = | ||
| - | == | + | == Office work == |
This is our first full week to work on this amazing project. There are four of us who can work whole days (Robert, Jeroen, Kees and Mark) and two of us who can work in the afternoon (Thijs and Wouter), the others will start when the sixth period end of our academic year. | This is our first full week to work on this amazing project. There are four of us who can work whole days (Robert, Jeroen, Kees and Mark) and two of us who can work in the afternoon (Thijs and Wouter), the others will start when the sixth period end of our academic year. | ||
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written by: Mark | written by: Mark | ||
| - | == | + | == Lab work == |
'''Growing cells''' | '''Growing cells''' | ||
| - | + | Monday: | |
''E. coli'' in liquid LB | ''E. coli'' in liquid LB | ||
Revision as of 10:04, 5 June 2012
week 2: 7 may - 11 may
Office work
This is our first full week to work on this amazing project. There are four of us who can work whole days (Robert, Jeroen, Kees and Mark) and two of us who can work in the afternoon (Thijs and Wouter), the others will start when the sixth period end of our academic year.
This week we searched for programs which can predict the tertary structure of the monomers of the VLPs, we found several of them, but phyre2 really stand out with the simple fill in sheat and the possibility to download the PDB files of your predicted protein. Besides searching for programs, we also worked on the protocols we are going to use and check if we've got the required equipment and materials. Thijs and Mark worked also on the logo for the team and the backbone for the wiki.
[Meeting]
written by: Mark
Lab work
Growing cells
Monday:
E. coli in liquid LB
3 plates per strain were picked and grown in 25 ml LB. all plates grown well accept for amp plates, due to drying effects.
Tuesday:
10:00 Continue growth. All 3 strains in shaker, 180 RPM, 30 degree celcius.
16:55
culture mach1 and DH5X are plated 10x and 1000x diluted on LB plates. Dilutions are done with demi water.
Plates were placed in 37 degree Celcius stove
Wensday:
9:15 10x dilution was to low, overgrown 1000x was nearly overrown, but still acceptable.
picked 1 MACH1 and 1 DH5X colony and grown in 10 ml SOB medium, 37 degree Celcius, 180 RPM
Making competent cells
Thursday:
MACH1 competent cells followin pinky's protocol. with the following diviations
- 4x 500 ml SOB
- SLA 3000 rotor
OD harvest at: 0.5 -0.45
Testing CCMV protocol
Friday:
CCMV protocol inoculated at 8:45 IPTG at 11:45
centrifugation in greiner tubes
pellet stored at -20 degree Celcius, 50 ml greiner tubes
Miniprep following the Gene Jet Fermentas protocol
- pet28
- standard 10 stock 1
- standard 10 stock 7
