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Team:Wageningen UR/Journal/week6
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The early monday of this week started with a bit of a shock, our plates with our transformed ''E.coli'' we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed ''E.coli'', we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks. | The early monday of this week started with a bit of a shock, our plates with our transformed ''E.coli'' we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed ''E.coli'', we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks. | ||
Revision as of 07:09, 5 June 2012
week 5: 4 june - 8 june
office work
This monday we've worked further on updating the wiki, we are now on scedule with the notebook and protocols. We also made a master plan on how the wiki should look like. The first steps are being made to create a prize winning wiki page.
[Meeting]
written by: Mark
lab work
Monday:
CCMV Bricking:
The early monday of this week started with a bit of a shock, our plates with our transformed E.coli we're almost complete empty. Luckely there were at least one colony of both of the with bricks transformed E.coli, we put them in Greiner tubes and after a few hours checked their OD. In the afternoon we prepared the samples for PCR to validate the bricks.
CCMV test protocol:
We've also made different samples ready for dialisis. There is one modification used in the protocol, instead of using the horrible sonifier, we used the French press, which is a great apperatus and easy to use. In the late afternoon we started with the dialysis.
