Team:Osaka/week5

From 2012.igem.org

(Difference between revisions)
Line 9: Line 9:
==August 27 (Mon)==
==August 27 (Mon)==
-
 
+
# miniprep(1-1G,1-5A)
 +
# Transformation(155,156)
 +
#*Transformed cells did not grow on plates
==August 28 (Tue)==
==August 28 (Tue)==
-
 
+
# Preparation of LB agar plates (Amp,Cam)
 +
# Ligation(155,156)
==August 29 (Wed)==
==August 29 (Wed)==

Revision as of 05:16, 28 August 2012

August 26 (Sun)

  1. Colony check
    • 1-1G,1-5A → Successfully transformed
    • 5-23O,1-7A → Transformed cells didn't grow on plates
  2. Transfer to liquid culture:1-1G,1-5A
  3. Preparation of LB agar plates (Tet)

August 27 (Mon)

  1. miniprep(1-1G,1-5A)
  2. Transformation(155,156)
    • Transformed cells did not grow on plates

August 28 (Tue)

  1. Preparation of LB agar plates (Amp,Cam)
  2. Ligation(155,156)

August 29 (Wed)

August 30 (Thu)

August 31 (Fri)

September 1 (Sat)

Back to Notebook