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	= week 2: 7 may - 11 may =		= week 2: 7 may - 11 may =
		+
		+	== office work ==
		+
		+	== lab work ==
		+
		+	7 may
		+
		+	''E. coli'' in liquid LB
		+
		+	3 plates per strain were picked and grown in 25 ml LB.
		+	all plates grown well accept for amp plates, due to drying effects.
		+
		+
		+	8 may
		+
		+	10:00
		+	Continue growth.
		+	All 3 strains in shaker, 180 RPM, 30 degree celcius.
		+
		+	4 x 1 L SOB is made
		+
		+	16:55
		+	culture mach1 and DH5X are plated 10x and 1000x diluted on LB plates. Dilutions are done with demi water.
		+
		+	Plates were placed in 37 degree Celcius stove
		+
		+
		+	9 may
		+
		+	9:15
		+	10x dilution was to low, overgrown
		+	1000x was nearly overrown, but still acceptable.
		+
		+	picked 1 MACH1 and 1 DH5X colony and grown in 10 ml SOB medium, 37 degree Celcius, 180 RPM
		+
		+
		+	10 may
		+
		+	MACH1 competent cells
		+	followin pinky's protocol. with the following diviations
		+	*4x 500 ml SOB
		+	*SLA 3000 rotor
		+
		+	OD harvest at: 0.5 -0.45
		+
		+
		+	11 may
		+
		+	CCMV protocol
		+	inoculated at 8:45 IPTG at 11:45
		+
		+	centrifugation in greiner tubes
		+
		+	pellet stored at -20 degree Celcius, 50 ml greiner tubes

---

Revision as of 07:31, 31 May 2012

week 2: 7 may - 11 may

office work

lab work

7 may

E. coli in liquid LB

3 plates per strain were picked and grown in 25 ml LB. all plates grown well accept for amp plates, due to drying effects.

8 may

10:00 Continue growth. All 3 strains in shaker, 180 RPM, 30 degree celcius.

4 x 1 L SOB is made

16:55 culture mach1 and DH5X are plated 10x and 1000x diluted on LB plates. Dilutions are done with demi water.

Plates were placed in 37 degree Celcius stove

9 may

9:15 10x dilution was to low, overgrown 1000x was nearly overrown, but still acceptable.

picked 1 MACH1 and 1 DH5X colony and grown in 10 ml SOB medium, 37 degree Celcius, 180 RPM

10 may

MACH1 competent cells followin pinky's protocol. with the following diviations

• 4x 500 ml SOB
• SLA 3000 rotor

OD harvest at: 0.5 -0.45

11 may

CCMV protocol inoculated at 8:45 IPTG at 11:45

centrifugation in greiner tubes

pellet stored at -20 degree Celcius, 50 ml greiner tubes