Revision as of 05:22, 18 September 2012

Wageningen UR

week 9: 25 june - 1 july

Biobrick BBa_B0015 (a terminator) was cloned.

TuYV

Wednesday:

Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells.

Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1, 1H and 2 plate.

Friday:

Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed on the agrose gel later. No positive results were acquired.

Written by: Han Yue

@@ Line 16: / Line 16: @@
 Wednesday:
-*Last week's PCR products were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells.
+*Last week's PCR products(1,1H,2,2H,3,3H) were digested, ligated into the linearized pSB1A3 backbone and transformed into our electrocompetent DH5 Alfa cells.
+Then after transformation, we checked the colonies on the plate, but only few colonies on positive control and sample 1, 1H and 2 plate.
 Friday:
-*A colony PCR was performed on the transformants. No positive results were acquired.
+*Colonies from 1, 1H and 2 plate were picked and checked by colony PCR. But no bands were showed on the agrose gel later. No positive results were acquired.
-Written by: Wouter
+Written by: Han Yue
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