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Team:Osaka/week2
From 2012.igem.org
(Difference between revisions)
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==August 6 (Mon)== | ==August 6 (Mon)== | ||
| + | # Miniprep of yesterday's culture | ||
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 2). | ||
| + | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
| + | |+Table2 | ||
| + | !ID!!Part Name!!Resistance!!Description | ||
| + | |- | ||
| + | |1-2D||<bbpart>BBa_J52008</bbpart>||A,K|| Renilla Luciferase | ||
| + | |- | ||
| + | |1-10H||<bbpart>BBa_J712019</bbpart>||A,K|| Firefly Luciferase | ||
| + | |- | ||
| + | |1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter | ||
| + | |} | ||
Revision as of 05:39, 17 August 2012
August 5 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transfer to liquid culture
- (Rosetta)143~146,149,152
- (DH5α)150,Empty vector(A)
August 6 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformaton of Registry parts into DH5α E.coli(See Table 2).
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-2D | <bbpart>BBa_J52008</bbpart> | A,K | Renilla Luciferase |
| 1-10H | <bbpart>BBa_J712019</bbpart> | A,K | Firefly Luciferase |
| 1-22A | <bbpart>BBa_J23118</bbpart> | A | Constitutive promoter |
