Team:Wageningen UR/Protocol/SDSPolyacrylamide

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= Purification of the VLPs =
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= SDS-polyacrylamide and native gel electrophoresis =
== Reagents & Materials ==
== Reagents & Materials ==
== Procedure ==
== Procedure ==
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*Wear gloves
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*Clean glass slides with MQ and dry
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<ol>
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<li>Prepare running gel mix in a small beaker for 2 gels:</li>
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*4 ml MQ
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*3.3 ml 30% acrylamide mix
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*2.5 ml 1.5 M TRIS pH 8.8
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*0.1 ml 10% SDS
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*0.1 ml 10% ammonium persulphate
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<li>Places glass slides in holders and tighten in rack.</li>
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''polymerization''
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<li>Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.</li>
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<li>Add a layer of iso-propanol on top of the polymerizing gel to equalize.</li>

Revision as of 11:29, 30 May 2012


SDS-polyacrylamide and native gel electrophoresis

Reagents & Materials

Procedure

  • Wear gloves
  • Clean glass slides with MQ and dry
  1. Prepare running gel mix in a small beaker for 2 gels:
    • 4 ml MQ
    • 3.3 ml 30% acrylamide mix
    • 2.5 ml 1.5 M TRIS pH 8.8
    • 0.1 ml 10% SDS
    • 0.1 ml 10% ammonium persulphate
  2. Places glass slides in holders and tighten in rack.

    3. polymerization

  3. Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.
  4. Add a layer of iso-propanol on top of the polymerizing gel to equalize.
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