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	== General Protocol ==		== General Protocol ==
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		+	*[[Team:Wageningen_UR/Protocol/ElectrocompetentCells|Preparation of electrocompetent cells of ''E. coli'']]

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Revision as of 09:27, 30 May 2012

Contents 1 Protocol 1.1 Medium & Buffer recipies 1.2 CCMV Coat Protein VLP formation 1.3 Hepatitis B Coat Protein VLP formation 1.4 PoLeRo Coat Protein VLP formation 1.5 General Protocol

Protocol

Medium & Buffer recipies

• LB medium
• SOB medium
• Disassembly buffer
• Dialysis buffer 10x
• Reassembly buffer
• Virus buffer

CCMV Coat Protein VLP formation

• Growing culture
• Dialysis of the VLPs
• Purifing the VLPs

Hepatitis B Coat Protein VLP formation

PoLeRo Coat Protein VLP formation

General Protocol

• Preparation of electrocompetent cells of E. coli