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Team:NTNU Trondheim/Notebook
From 2012.igem.org
(Difference between revisions)
(→Thursday 09.08.12) |
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| - | Did a restriction digest as described in the protocol on the following parts. | + | Did a restriction digest as described in the [https://2012.igem.org/Team:NTNU_Trondheim/Protocols protocol] on the following parts. On the pieces to be used as inserts, the number of base pairs are also listed. |
{| class="wikitable" style="text-align:center;margin: 1em auto 1em auto;" | {| class="wikitable" style="text-align:center;margin: 1em auto 1em auto;" | ||
| Line 19: | Line 19: | ||
!Enzymes | !Enzymes | ||
!Buffer | !Buffer | ||
| + | !bp insert | ||
|- | |- | ||
|LuxI+term | |LuxI+term | ||
| Line 24: | Line 25: | ||
|XbaI+PstI | |XbaI+PstI | ||
|3 | |3 | ||
| + | |747 | ||
| + | |- | ||
| + | |YFP+term | ||
| + | |<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo> | ||
| + | |XbaI+PstI | ||
| + | |3 | ||
| + | |852 | ||
|- | |- | ||
|Lysis | |Lysis | ||
| Line 29: | Line 37: | ||
|XbaI+PstI | |XbaI+PstI | ||
|3 | |3 | ||
| + | |1785 | ||
|- | |- | ||
|LuxI | |LuxI | ||
| Line 34: | Line 43: | ||
|XbaI+PstI | |XbaI+PstI | ||
|3 | |3 | ||
| + | |618 | ||
|- | |- | ||
|RBS* | |RBS* | ||
| Line 39: | Line 49: | ||
|SpeI+PstI | |SpeI+PstI | ||
|1 | |1 | ||
| + | | - | ||
|- | |- | ||
|P<sub>luxR+HSL</sub> | |P<sub>luxR+HSL</sub> | ||
| Line 44: | Line 55: | ||
|SpeI+PstI | |SpeI+PstI | ||
|1 | |1 | ||
| + | | - | ||
|- | |- | ||
|RBS | |RBS | ||
| Line 49: | Line 61: | ||
|SpeI+PstI | |SpeI+PstI | ||
|1 | |1 | ||
| + | | - | ||
|- | |- | ||
|pSB1A3 | |pSB1A3 | ||
| Line 54: | Line 67: | ||
|EcoRI+PstI+DpnI | |EcoRI+PstI+DpnI | ||
|3 | |3 | ||
| + | | - | ||
|- | |- | ||
|plld | |plld | ||
| Line 59: | Line 73: | ||
|EcoRI+SpeI | |EcoRI+SpeI | ||
|4 | |4 | ||
| + | |433 | ||
|- | |- | ||
|} | |} | ||
| - | The parts lysis, YFP+term, pSB1A3 and plld will be assembled using the 3A assembly, made double cutting mix | + | The parts lysis <partinfo>BBa_K112808</partinfo>, YFP+term (<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>), <partinfo>pSB1A3</partinfo> and plld will be assembled using the 3A assembly, so made double cutting mix with these parts. |
| + | |||
| + | Gel electrophoresis was done with luxI+term (<partinfo>Bba_C0061</partinfo>, <partinfo>BBa_B0015</partinfo>), lysis <partinfo>BBa_K112808</partinfo>, YFP+term (<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>), LuxI <partinfo>Bba_C0061</partinfo> and plld. | ||
===Wednesday 08.08.12=== | ===Wednesday 08.08.12=== | ||
Revision as of 12:31, 9 August 2012
