Team:Cambridge/Lab book/Week 7

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===Tuesday===
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'''[[zzz|Production of electrocompetent e.coli]]'''
===Wednesday===
===Wednesday===

Revision as of 10:48, 7 August 2012

Week: 3 4 5 6 7

Contents

Monday

PCR of Magnesium riboswitch vector fragment B and Magnesium promoter

Riboswitch and promoter gel. Lanes 2+3: Fragment B without 8 codon substitution, lanes 4+5: Fragment B with 8 codon substitution, lanes 6+7: magnesium sensitive promoter, lane 8: +ve control
  • Normal PCR settings used, annealing temperature 57 °C, elongation step 90s long.
  • Lane 5 accidentally loaded with a DNA ladder instead of loading dye.
  • Expected fragment sizes:
  • Lane 2-5: 3kbp
  • Lane 6-7: 300bp
  • After electrophoresis, found vector products had, for the most part, worked. Promoter elements were not amplified - no band of the expected size was observed.
  • Positive control also failed, although this has ceased to work for several days, potentially due to DNA degradation.

Tuesday

Production of electrocompetent e.coli

Wednesday

Thursday

Friday

Retrieved from "http://2012.igem.org/Team:Cambridge/Lab_book/Week_7"