Team:Evry/Protocols

From 2012.igem.org

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<h1>PCR with Phusion polymerase<h1>
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<u>Préparation LB et PB Agar:</u>
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<h1>Préparation of LB medium and LB Agar:</h1>
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<b>=> LB Agar :</b>
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<b>=> Lb Agar :</b>
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-18,5g LB Agar
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-18,5g de LB Agar
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-300ml H2O
-300ml H2O
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<b>=> LB medium :</b>
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-6g LB broth
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-300ml de H2O
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Autoclaved at 250°C
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<b>=> LB :</b>
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-
-6g de LB broth
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-
-300ml de H2O
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-
-Autoclave
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Revision as of 12:31, 3 August 2012

PCR with Phusion polymerase<h1> <h1>Préparation of LB medium and LB Agar:


=> LB Agar : -18,5g LB Agar -300ml H2O

=> LB medium : -6g LB broth -300ml de H2O

Autoclaved at 250°C














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