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Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
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</td><td>for BB detection when sequenced | </td><td>for BB detection when sequenced | ||
| + | <h1>Plasmids</h1> | ||
</table> | </table> | ||
Revision as of 15:37, 1 August 2012
Freezer
Primers
| Number | Name | Sequence | Length | Tm | %GC | Comments |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | |
| P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | |
| P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | 21 | 54 | 48 | |
| P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | 40 | 74 | 58 | |
| P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | 51 | 70 | 43 | |
| P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | 30 | 67 | 53 | Heat Shock |
| P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | 31 | 63 | 42 | Heat Shock |
| P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | 27 | 68 | 63 | Vasculature |
| P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | 31 | 64 | 45 | Vasculature |
| P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | 25 | 61 | 52 | Ubiquitous |
| P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | 27 | 66 | 56 | Ubiquitous |
| P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | 32 | 66 | 47 | muscles |
| P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | 30 | 63 | 43 | muscles |
| P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | 19 | 55 | 47 | |
| P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | 25 | 52 | 28 | |
| P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | 20 | 56 | 50 | |
| P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | 18 | 55 | 56 | |
| P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | 18 | 64 | 72 | |
| P24 | Sp6 | ATTTAGGTGACACTATA | 17 | 41 | 27 | for BB detection when sequenced |
| P25 | T3 | AATTAACCCTCACTAAAGGG | 20 | 50 | 40 | for BB detection when sequenced
Plasmids |
