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Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
| Line 116: | Line 116: | ||
</td><td>pCS2+BB-Rv | </td><td>pCS2+BB-Rv | ||
</td><td>tgcatGAATTCGAATCGATGGGATCC | </td><td>tgcatGAATTCGAATCGATGGGATCC | ||
| - | </td><td> | + | </td><td>21 |
| - | </td><td> | + | </td><td>54 |
| - | </td><td> | + | </td><td>48 |
</td><td> | </td><td> | ||
| Line 125: | Line 125: | ||
</td><td>GFP-AID-BB-Fw | </td><td>GFP-AID-BB-Fw | ||
</td><td>gaattcgcggccgcttctagagATGGCCACAACCATGGTG | </td><td>gaattcgcggccgcttctagagATGGCCACAACCATGGTG | ||
| - | </td><td> | + | </td><td>40 |
| - | </td><td> | + | </td><td>74 |
| - | </td><td> | + | </td><td>58 |
</td><td> | </td><td> | ||
| Line 134: | Line 134: | ||
</td><td>GFP-AID-BB-Rv | </td><td>GFP-AID-BB-Rv | ||
</td><td>tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | </td><td>tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | ||
| - | </td><td> | + | </td><td>51 |
| - | </td><td> | + | </td><td>70 |
| - | </td><td> | + | </td><td>43 |
</td><td> | </td><td> | ||
| Line 143: | Line 143: | ||
</td><td>p2 Hsp70-Fw | </td><td>p2 Hsp70-Fw | ||
</td><td>ACTGTCGACCCCGTTTAGCAGGAAATAGCC | </td><td>ACTGTCGACCCCGTTTAGCAGGAAATAGCC | ||
| - | </td><td> | + | </td><td>30 |
| - | </td><td> | + | </td><td>67 |
| - | </td><td> | + | </td><td>53 |
| - | </td><td> | + | </td><td>Heat Shock |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 152: | Line 152: | ||
</td><td>p2 Hsp70-Rv | </td><td>p2 Hsp70-Rv | ||
</td><td>ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | </td><td>ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | ||
| - | </td><td> | + | </td><td>31 |
| - | </td><td> | + | </td><td>63 |
| - | </td><td> | + | </td><td>42 |
| - | </td><td> | + | </td><td>Heat Shock |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 161: | Line 161: | ||
</td><td>p2 Flk-1-Fw | </td><td>p2 Flk-1-Fw | ||
</td><td>ACTGTCGACCTGGACTCTGGGCTAGGG | </td><td>ACTGTCGACCTGGACTCTGGGCTAGGG | ||
| - | </td><td> | + | </td><td>27 |
| - | </td><td> | + | </td><td>68 |
| - | </td><td> | + | </td><td>63 |
| - | </td><td> | + | </td><td>Vasculature |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 170: | Line 170: | ||
</td><td>p2 Flk-1-Rv | </td><td>p2 Flk-1-Rv | ||
</td><td>TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | </td><td>TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | ||
| - | </td><td> | + | </td><td>31 |
| - | </td><td> | + | </td><td>64 |
| - | </td><td> | + | </td><td>45 |
| - | </td><td> | + | </td><td>Vasculature |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 179: | Line 179: | ||
</td><td>p2 ROSA-Fw | </td><td>p2 ROSA-Fw | ||
</td><td>ACTGTCGACTAGATGAAGGAGAGCC | </td><td>ACTGTCGACTAGATGAAGGAGAGCC | ||
| - | </td><td> | + | </td><td>25 |
| - | </td><td> | + | </td><td>61 |
| - | </td><td> | + | </td><td>52 |
| - | </td><td> | + | </td><td>Ubiquitous |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 188: | Line 188: | ||
</td><td>p2 ROSA-Rv | </td><td>p2 ROSA-Rv | ||
</td><td>GATAAGCTTGGATCCCCGCAAACGCAC | </td><td>GATAAGCTTGGATCCCCGCAAACGCAC | ||
| - | </td><td> | + | </td><td>27 |
| - | </td><td> | + | </td><td>66 |
| - | </td><td> | + | </td><td>56 |
| - | </td><td> | + | </td><td>Ubiquitous |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 197: | Line 197: | ||
</td><td>p2 CarA-Fw | </td><td>p2 CarA-Fw | ||
</td><td>ACTGTCGACGTCCCATACCAGTACAATGCAAT | </td><td>ACTGTCGACGTCCCATACCAGTACAATGCAAT | ||
| - | </td><td> | + | </td><td>32 |
| - | </td><td> | + | </td><td>66 |
| - | </td><td> | + | </td><td>47 |
| - | </td><td> | + | </td><td>muscles |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 206: | Line 206: | ||
</td><td>p2 CarA-Rv | </td><td>p2 CarA-Rv | ||
</td><td>AGTAAGCTTGTGCTGTGATTGAATTGGCTG | </td><td>AGTAAGCTTGTGCTGTGATTGAATTGGCTG | ||
| - | </td><td> | + | </td><td>30 |
| - | </td><td> | + | </td><td>63 |
| - | </td><td> | + | </td><td>43 |
| - | </td><td> | + | </td><td>muscles |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 215: | Line 215: | ||
</td><td>IaaH-BB-Fw | </td><td>IaaH-BB-Fw | ||
</td><td>gaattcgcggccgcttctagATGCGCGAAATGATTACGC | </td><td>gaattcgcggccgcttctagATGCGCGAAATGATTACGC | ||
| - | </td><td> | + | </td><td>19 |
| - | </td><td> | + | </td><td>55 |
| - | </td><td> | + | </td><td>47 |
</td><td> | </td><td> | ||
| Line 224: | Line 224: | ||
</td><td>IaaH-BB-Rv | </td><td>IaaH-BB-Rv | ||
</td><td>ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | </td><td>ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | ||
| - | </td><td> | + | </td><td>25 |
| - | </td><td> | + | </td><td>52 |
| - | </td><td> | + | </td><td>28 |
</td><td> | </td><td> | ||
| Line 233: | Line 233: | ||
</td><td>IaaM-BB-Fw | </td><td>IaaM-BB-Fw | ||
</td><td>gaattcgcggccgcttctagatgtttggaccggatttccc | </td><td>gaattcgcggccgcttctagatgtttggaccggatttccc | ||
| - | </td><td> | + | </td><td>20 |
| - | </td><td> | + | </td><td>56 |
| - | </td><td> | + | </td><td>50 |
</td><td> | </td><td> | ||
| Line 242: | Line 242: | ||
</td><td>IaaM-BB-Rv | </td><td>IaaM-BB-Rv | ||
</td><td>ctgcagcggccgctactagtattattagtcccccagcgc | </td><td>ctgcagcggccgctactagtattattagtcccccagcgc | ||
| - | </td><td> | + | </td><td>18 |
| - | </td><td> | + | </td><td>55 |
| - | </td><td> | + | </td><td>56 |
</td><td> | </td><td> | ||
| Line 251: | Line 251: | ||
</td><td>AID-Fw | </td><td>AID-Fw | ||
</td><td>cgcttctagGGAGCTGGTGCAGGCGCT | </td><td>cgcttctagGGAGCTGGTGCAGGCGCT | ||
| - | </td><td> | + | </td><td>18 |
| - | </td><td> | + | </td><td>64 |
| - | </td><td> | + | </td><td>72 |
</td><td> | </td><td> | ||
| Line 260: | Line 260: | ||
</td><td>Sp6 | </td><td>Sp6 | ||
</td><td>ATTTAGGTGACACTATA | </td><td>ATTTAGGTGACACTATA | ||
| - | </td><td> | + | </td><td>17 |
| - | </td><td> | + | </td><td>41 |
| - | </td><td> | + | </td><td>27 |
| - | </td><td> | + | </td><td>for BB detection when sequenced |
<tr style="background: #cccccc;"> | <tr style="background: #cccccc;"> | ||
| Line 269: | Line 269: | ||
</td><td>T3 | </td><td>T3 | ||
</td><td>AATTAACCCTCACTAAAGGG | </td><td>AATTAACCCTCACTAAAGGG | ||
| - | </td><td> | + | </td><td>20 |
| - | </td><td> | + | </td><td>50 |
| - | </td><td> | + | </td><td>40 |
| - | </td><td> | + | </td><td>for BB detection when sequenced |
</table> | </table> | ||
Revision as of 15:34, 1 August 2012
Freezer
Primers
| Number | Name | Sequence | Length | Tm | %GC | Comments |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | |
| P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | |
| P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | 21 | 54 | 48 | |
| P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | 40 | 74 | 58 | |
| P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | 51 | 70 | 43 | |
| P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | 30 | 67 | 53 | Heat Shock |
| P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | 31 | 63 | 42 | Heat Shock |
| P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | 27 | 68 | 63 | Vasculature |
| P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | 31 | 64 | 45 | Vasculature |
| P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | 25 | 61 | 52 | Ubiquitous |
| P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | 27 | 66 | 56 | Ubiquitous |
| P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | 32 | 66 | 47 | muscles |
| P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | 30 | 63 | 43 | muscles |
| P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | 19 | 55 | 47 | |
| P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | 25 | 52 | 28 | |
| P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | 20 | 56 | 50 | |
| P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | 18 | 55 | 56 | |
| P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | 18 | 64 | 72 | |
| P24 | Sp6 | ATTTAGGTGACACTATA | 17 | 41 | 27 | for BB detection when sequenced |
| P25 | T3 | AATTAACCCTCACTAAAGGG | 20 | 50 | 40 | for BB detection when sequenced
|
