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From 2012.igem.org

Revision as of 15:22, 1 August 2012 (view source) Joachim (Talk | contribs) ← Older edit		Revision as of 15:23, 1 August 2012 (view source) Joachim (Talk | contribs) Newer edit →
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	<td style="background: #ccffcc;">P7		<td style="background: #ccffcc;">P7
	</td><td>pCS2+BB-Fw		</td><td>pCS2+BB-Fw
-	</td><tdcgctgcagGAACTATAGTGAGTCGTATTACG	+	</td><td>cgctgcagGAACTATAGTGAGTCGTATTACG
	</td><td>23		</td><td>23
	</td><td>51		</td><td>51

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Revision as of 15:23, 1 August 2012

Freezer

Primers

Number	Name	Sequence	Length	Tm	%GC	Comments
P1	Tir1-BB-FW	cgcttctagATGACGTACTTCCCGGAGG	19	57	58
P2	Tir1-BB-Rv	ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC	24	52	29
P3	SdMTir1-BB-Fw	AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG	65	72	43
P4	SdMTir1-BB-Rv	TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG	62	76	52
P5	Tir1 SdM v02-Fw	tgcTgtgacagaggagggac	20	60	60
P6	Tir1 SdM v02-Fw	ctagAgcagcattagtcatttgg	23	55	43
P7	pCS2+BB-Fw	cgctgcagGAACTATAGTGAGTCGTATTACG	23	51	39
P8	pCS2+BB-Rv	tgcatGAATTCGAATCGATGGGATCC
P9	GFP-AID-BB-Fw	gaattcgcggccgcttctagagATGGCCACAACCATGGTG
P10	GFP-AID-BB-Rv	tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC
P11	p2 Hsp70-Fw	ACTGTCGACCCCGTTTAGCAGGAAATAGCC
P12	p2 Hsp70-Rv	ACTAAGCTTATTTGCGCTCCTTACAGTTTGC
P13	p2 Flk-1-Fw	ACTGTCGACCTGGACTCTGGGCTAGGG
P14	p2 Flk-1-Rv	TCAAAGCTTGTTGACGTTTAGTCCAGGACAG
P15	p2 ROSA-Fw	ACTGTCGACTAGATGAAGGAGAGCC
P16	p2 ROSA-Rv	GATAAGCTTGGATCCCCGCAAACGCAC
P17	p2 CarA-Fw	ACTGTCGACGTCCCATACCAGTACAATGCAAT
P18	p2 CarA-Rv	AGTAAGCTTGTGCTGTGATTGAATTGGCTG
P19	IaaH-BB-Fw	gaattcgcggccgcttctagATGCGCGAAATGATTACGC
P20	IaaH-BB-Rv	ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG
P21	IaaM-BB-Fw	gaattcgcggccgcttctagatgtttggaccggatttccc
P22	IaaM-BB-Rv	ctgcagcggccgctactagtattattagtcccccagcgc
P23	AID-Fw	cgcttctagGGAGCTGGTGCAGGCGCT
P24	Sp6
P25	T3