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Team:Cambridge/Lab book/Week 6
From 2012.igem.org
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Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | ||
| - | PCR cycle x35 : 15s Denaturing at 95 C | + | PCR cycle x35 : *15s Denaturing at 95 C |
| - | 45s Annealing at 60 C | + | *45s Annealing at 60 C |
| - | 300s Extension at 72 C | + | *300s Extension at 72 C |
===Tuesday=== | ===Tuesday=== | ||
Revision as of 10:44, 30 July 2012
| Week: | 3 | 4 | 5 | 6 | 7 |
|---|
Contents |
Monday
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
PCR cycle x35 : *15s Denaturing at 95 C
*45s Annealing at 60 C
*300s Extension at 72 C
Tuesday
Wednesday
Thursday
Friday
