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Team:TU-Eindhoven
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This year's project will be all about [https://2012.igem.org/Team:TU-Eindhoven/LEC '''Light Emitting Cells'''] (LECs) that emit light in response to an electric stimulus. A LEC has to emit light conditionally, that is, it has to react a control signal. In our case the control signal will be an electric field that is strong enough to depolarize the plasma membrane of ''Saccharomyces cerevisiae'' cells, but weak enough for the cells to survive. | This year's project will be all about [https://2012.igem.org/Team:TU-Eindhoven/LEC '''Light Emitting Cells'''] (LECs) that emit light in response to an electric stimulus. A LEC has to emit light conditionally, that is, it has to react a control signal. In our case the control signal will be an electric field that is strong enough to depolarize the plasma membrane of ''Saccharomyces cerevisiae'' cells, but weak enough for the cells to survive. | ||
| + | {{:Team:TU-Eindhoven/Templates/h2|header=Light Emitting Cells}} | ||
The plasma membrane of ''S. cerevisiae'' contains the CCH1-MID1 channel protein complex that is homologous to mammalian Voltage Gated Calcium Channels (VGCCs). It is hypothesized that upon depolarization of the plasma membrane, calcium ions selectively enter the cytoplasm through CCH1-MID1. Light will be emitted through the fluorescence of GECO protein, a calcium sensor that is expressed from an genetically engineered plasmid. When the calcium concentration is high the GECO proteins will be fluorescent, when the calcium concentration is low the GECO proteins will not be fluorescent. After a while the calcium concentration will drop to homeostatic levels through active transport to the yeast's vacuole and fluorescence will cease. | The plasma membrane of ''S. cerevisiae'' contains the CCH1-MID1 channel protein complex that is homologous to mammalian Voltage Gated Calcium Channels (VGCCs). It is hypothesized that upon depolarization of the plasma membrane, calcium ions selectively enter the cytoplasm through CCH1-MID1. Light will be emitted through the fluorescence of GECO protein, a calcium sensor that is expressed from an genetically engineered plasmid. When the calcium concentration is high the GECO proteins will be fluorescent, when the calcium concentration is low the GECO proteins will not be fluorescent. After a while the calcium concentration will drop to homeostatic levels through active transport to the yeast's vacuole and fluorescence will cease. | ||
Revision as of 20:55, 15 July 2012
The Project
This year's project will be all about Light Emitting Cells (LECs) that emit light in response to an electric stimulus. A LEC has to emit light conditionally, that is, it has to react a control signal. In our case the control signal will be an electric field that is strong enough to depolarize the plasma membrane of Saccharomyces cerevisiae cells, but weak enough for the cells to survive.
Light Emitting Cells
The plasma membrane of S. cerevisiae contains the CCH1-MID1 channel protein complex that is homologous to mammalian Voltage Gated Calcium Channels (VGCCs). It is hypothesized that upon depolarization of the plasma membrane, calcium ions selectively enter the cytoplasm through CCH1-MID1. Light will be emitted through the fluorescence of GECO protein, a calcium sensor that is expressed from an genetically engineered plasmid. When the calcium concentration is high the GECO proteins will be fluorescent, when the calcium concentration is low the GECO proteins will not be fluorescent. After a while the calcium concentration will drop to homeostatic levels through active transport to the yeast's vacuole and fluorescence will cease.In conclusion: An electric field is used to control the fluorescence of a calcium sensor protein in a yeast cell.
Device
Introduction to the device for stimulation of LECs.Achievements in iGEM competition
- TODO
Please see iGEM official results page to see how all the teams did.
In the news
- TODO
The team - in order - Member A, Member B, Member C.
