Team:UPIBI-Mexico/Project

From 2012.igem.org

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<center><h1>Constitutive chimeric promoters for the expression of recombinant proteins</h1></center>
<center><h1>Constitutive chimeric promoters for the expression of recombinant proteins</h1></center>
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<p>Ever wanted to produce huge amount of your favorite recombinant protein? Here we will show you how!
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<p>Ever wanted to produce huge amount of your favorite recombinant protein? Here we will show you how!</p>  
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In this part of the project we aim to develop constitutive chimeric promoters that could allow for accumulation of different levels of recombinant proteins in Chlamydomonas reinhardtii chloroplast. The psbA promoter is one of the strongest promoters in green eukaryotic algae. This promoter has been used in the past (reference) to drive the expression of foreign proteins. Unfortunately, this promoter seems to render high level of protein accumulation only when the endogenous psbA product, the D1 protein, is absent. This has led some people to think that the D1 is somehow regulating the transcription of the ORF under the control of the psbA promoter.</p>
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<p>We think that since the psbA mRNA levels are fairly constant throughout the growing cycle, the D1 protein is actually regulating translation of the mRNA by interacting with the 5´UTR. We reasonedº that if we can relieve the mRNA from this control element, but keeping its transcription at high level, a higher level of recombinant protein accumulation could be achieved. We think that replacing the psbA 5´UTR with other 5´UTR that have been shown to work well in prokaryotes can do this. Given the similarities of the chloroplast with prokaryotes, the effectiveness of these 5´UTRs should remain to a certain extent.
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<div id=project2>
<div id=project2>
<center><h1>Regulated expression of recombinant proteins </h1></center>
<center><h1>Regulated expression of recombinant proteins </h1></center>
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<p>Tired of those easy to degrade yet expensive proteins? Try our regulate expression Chlamy system to turn on the expression only when you want it.</p>
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<div id=project3>
<div id=project3>
<center><h1>Regulated expression of enzymes contained in an operon</h1></center>
<center><h1>Regulated expression of enzymes contained in an operon</h1></center>
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<center><h1>Regulated expression of recombinant proteins </h1></center>
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<p>Imagine an illuminated or colorful world with algae that emit light or produce bright red carotenoids. Follow the light here!</p>
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Revision as of 03:09, 13 July 2012

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Main

Constitutive chimeric promoters for the expression of recombinant proteins

Ever wanted to produce huge amount of your favorite recombinant protein? Here we will show you how!

Regulated expression of recombinant proteins

Tired of those easy to degrade yet expensive proteins? Try our regulate expression Chlamy system to turn on the expression only when you want it.

Regulated expression of enzymes contained in an operon

Regulated expression of recombinant proteins

Imagine an illuminated or colorful world with algae that emit light or produce bright red carotenoids. Follow the light here!

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