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| | {{:Team:TU-Eindhoven/Templates/headerbar|colour=#386abc|title=The Project}} | | {{:Team:TU-Eindhoven/Templates/headerbar|colour=#386abc|title=The Project}} |
| - | <html><img src="https://static.igem.org/mediawiki/2010/7/70/Front.jpg" style="margin-left:4px; margin-bottom:5px; width:710px; margin-top:-2px;"> | + | <html><img src="/wiki/images/EindhovenBoxes.jpg" style="margin-left:4px; margin-bottom:5px; width:710px; margin-top:-2px;"> |
| | <div style="height:30px; position:relative; font-size:12px; color:#6e6e6e; line-height:15px;"> | | <div style="height:30px; position:relative; font-size:12px; color:#6e6e6e; line-height:15px;"> |
| - | <div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div> | + | <div style="position:absolute; width:230px; left:5px; text-align:center;">Description of box 1.</div> |
| - | <div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div> | + | <div style="position:absolute; width:230px; left:240px; text-align:center;">Description of box 2.</div> |
| - | <div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div> | + | <div style="position:absolute; width:220px; left:485px; text-align:center;">Description of box 3.</div> |
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| | </html> | | </html> |
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| - | Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices.
| + | TODO: Introduction to the project. |
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| - | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Project Firefly}} | + | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Light Emitting Cells}} |
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| - | We adopted a number of strategies to extend the use of '''firefly luciferase''':
| + | Some intro to '''this topic''': |
| - | * [https://2010.igem.org/Team:Cambridge/Codons '''Codon optimisation'''] for increased light output | + | * [/Team:TU-Eindhoven/LEC '''Light Emitting Cells'''] emit light in response to an electric stimulus. |
| - | * Use of a [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration '''luciferin regenerating enzyme'''].
| + | |
| - | * Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours''']
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| - | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Project Vibrio | + | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Device}} |
| - | }} | + | Introduction to the [/Team:TU-Eindhoven/Tools/Device '''device'''] for stimulation of LECs. |
| - | We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''. We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate.
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| - | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Tools}}
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| - | During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique.
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| - | Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
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| - | * [https://2010.igem.org/Team:Cambridge/Tools/Gibson '''Gibthon Construct Designer'''] allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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| - | * [https://2010.igem.org/Team:Cambridge/Tools/GenBank '''BioBrick → GenBank'''] allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
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| - | * The [https://2010.igem.org/Team:Cambridge/Tools/Ligate '''Ligation Calculator'''] is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
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| - | * The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors'''].
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| | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Achievements in iGEM competition | | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=Achievements in iGEM competition |
| | }} | | }} |
| - | | + | * TODO |
| - | * Finalist
| + | Please see [https://igem.org/Results '''iGEM official results page'''] to see how all the teams did. |
| - | * Winner of "Best Wiki" award (awarded jointly to Cambridge and [https://2010.igem.org/Team:Imperial_College_London '''Imperial College London'''])
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| - | * Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
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| - | * Awarded a Gold Medal | + | |
| - | Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did. | + | |
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| | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=In the news | | {{:Team:TU-Eindhoven/Templates/Nolineheader2|header=In the news |
| | }} | | }} |
| - | * [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article] | + | * TODO |
| - | * [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
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| - | * [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
| + | [[Image:EindhovenTeam.jpg|center|frame|The team - in order - Amoeba A, Amoeba B, Amoeba C.]] |
| | + | <html><a href="/Team:TU-Eindhoven/edit"> </a></div></html> |
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| - | [[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins.]]
| + | {{:Team:TU-Eindhoven/Templates/footerMinimal}} |
| - | <html><a href="/wiki/index.php?title=Team:TU-Eindhoven&action=edit"> </a></div></html>{{:Team:TU-Eindhoven/Templates/footerMinimal}}
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