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Team:Evry/Notebook/June/20
From 2012.igem.org
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Latest revision as of 13:05, 10 July 2012
Today's achievements:
Previous work : liquid culture of DH5a with mRFP and auxin plasmid
Main goals :
°Auxin toxicity test
°Bacterial integration assessment into the xenopus embryos
1°/Xenopus embryos presents a protective membrane, we decided to realize our bacterial deposits experiments on xenopus with and without their membrane. Then, we could see if bacterias could enter into the embryos naturally.
2°/ On 96 plates :
Plate 1
each plate contain : 1 embryo
Line A & B = on embryos without protective membrane : 500 uL mRFP plasmid on DH5a deposite + 1mL LB + 1mL MMR medium
Line C : 1 2 3 : normal embryos : 500 uL mRFP plasmid on DH5a deposite + 1mL (LB + MMR medium)
Line E & F = embryos without protective membrane : 500 uL AUX plasmid integrated on DH5a deposit + 1mL LB + 1mL MMR medium
Line G & M1-6 = normal embryos : 500 uL AUX plasmid integrated on DH5a deposit + 1mL LB + 1mL MMR medium
Line M7-9 = normal embryos : controls
Plate 2
normal embryos only
Line A & B
Line C : LB/MMR medium
Line D : RFP 0.1uL
Line E : AUX 0.1uL
Line G & H : LB/MMR medium only (no embryos)
3°/ On 16 Plates
Tadpoles experimentations :
each plate contains 3 tadpoles
Line A : 0.5uL RFP bacteria
Line B1 : 0.3uL RFP bacteria
We put this plate on UV light 15min after the deposit:
