Team/CINVESTAV-IPN-UNAM MX/home.htm

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<!--Fireworks CS6 Dreamweaver CS6 target.  Created Tue Sep 25 17:05:18 GMT-0500 (Central Daylight Time (Mexico)) 2012-->
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<li><a href="home.htm">Home</a></li>
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  <tr>
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<li><a href="#">Rhodofactory</a></li>
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  <td><img src="images/spacer.gif" width="240" height="1" alt="" /></td>
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<li><a href="#">Promoter selection </a></li>
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  <td><img src="images/spacer.gif" width="710" height="1" alt="" /></td>
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<li><a href="#">results </a></li>
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<a href="/Team/CINVESTAV-IPN-UNAM_MX/home.htm">Home<span class="flecha">&#9660;</span></a>
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<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Overview.htm">Overview<span class="flecha">&#9660;</span></a></li>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Light Response.htm">Light Response<span class="flecha">&#9660;</span></a></li>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Oxigen Response.htm">Oxigen Response<span class="flecha">&#9660;</span></a></li>
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<h1><em>Rhodofactory</em></h1>
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<h3>controlling genetic expression: an oxygen and light response </h3><br />
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<img src="https://static.igem.org/mediawiki/2012/c/ce/Imghome1.jpg" alt="" width="402" height="279" class="left" />
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      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria  allows them to grow in light, darkness and with or without oxygen; all  it is due to their genetic regulation mechanisms. Taking advantage of  this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis cluster regulation. </p>
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</li>
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  <p>The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion. </p>
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  <p>Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.   </p>
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<a href="">Results<span class="flecha">&#9660;</span></a>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/palustris response.htm">R. palustris response<span class="flecha">&#9660;</span></a></li>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Human Practice.htm">Human Practices<span class="flecha">&#9660;</span></a></li>
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      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis regulation. </p>
-
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
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      <p>The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on <em>R. palustris</em>. We built a genetic circuit that activate or repress GFP expression in response to external conditions.</p>
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<div id="sponsors">
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       <p> Once we characterize the functionality of these networks, we want to create a Rhodofactory to use our systems to control production butanol using simple signals. </p>
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  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="78" height="91" /></div>
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Revision as of 16:38, 22 October 2012

Home

Logo Principal

The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on R. sphaeroides photosynthesis regulation.

The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on R. palustris. We built a genetic circuit that activate or repress GFP expression in response to external conditions.

Once we characterize the functionality of these networks, we want to create a Rhodofactory to use our systems to control production butanol using simple signals.

Retrieved from "http://2012.igem.org/Team/CINVESTAV-IPN-UNAM_MX/home.htm"